2×PCR MasterMix
This premix contains all essential components for PCR: Taq DNA polymerase, dNTPs, MgCl₂, reaction buffer, PCR enhancer and electrophoresis tracking dye, supporting a wide range of PCR applications.
It offers simple operation: users only need to prepare templates and primers to run PCR assays. As a 2×premixed solution, it minimizes handling steps to reduce contamination risk and experimental errors. It features high amplification efficiency and sensitivity.
After amplification, the reaction product can be loaded directly for electrophoresis. The PCR product carries a terminal A-overhang, compatible with T‑vector cloning without extra A‑tailing treatment.
Package Specifications
| Component |
1 mL × 10 |
1 mL × 100 |
5 mL × 20 |
| 2× Taq PCR Mix (with tracking dye) |
1 mL × 10 |
1 mL × 100 |
5 mL × 20 |
| Instruction Manual |
1 copy |
1 copy |
1 copy |
Storage
Store at -20 ℃. Shelf life: 1 year.
Reaction Protocol (30 μL standard system)
Add the following reagents into a clean PCR tube:
| Component |
Dosage |
| 2× Taq PCR Mix (with tracking dye) |
15 μL |
| Template Mammalian genomic DNA |
0.5–1 μg |
| Yeast genomic DNA |
5–500 ng |
| Bacterial genomic DNA |
0.5–50 ng |
| Plasmid DNA |
5–500 pg |
| Purified PCR fragment |
1–100 pg |
| Each PCR primer (user-supplied) |
10 pmol |
| ddH₂O |
Adjust to 30 μL |
Run thermal cycling on a PCR instrument. After the reaction, take 5–10 μL for direct electrophoresis. No separate loading dye is required. The red tracking dye migrates at approximately the speed of a 50 bp DNA fragment.
Notes
- If the final reaction volume differs from 30 μL, adjust all components proportionally.
- When templates contain unknown PCR inhibitors (common in plant, soil and blood DNA samples), 1/10 PCR Inhibitor Remover (Cat.No.: BTN60804, add 3 μL into 30 μL system) may improve amplification performance.
