• Proteinase K(NGS)
  • Proteinase K(NGS)

Proteinase K(NGS)

ource: Tritirachiumalbum; Classification: EC 3.4.21.64; Molecular weight: 29 kDa (SDS-PAGE); Isoelectric point: 7.81; The optimal pH ranges from 7.0 to 12.0, indicating high activity; The optimal temperature is 65 ℃; PH stability pH 4.5-12.5 (25 ℃, 16 h); Thermal stability: Stable below 50 ℃ (pH 8.0,30 min), storage stability: Over 90% activity per year at 25 ℃, activator SDS, urea inhibitor diisopropylfluorophosphate (DFIP), benzoyl fluoride (PMSF); Storage conditions: Dry powder state -20 ℃
$45.00
  • Proteinase K(NGS)

SPECIFICATION

Protease K is a serine protease with efficient enzyme activity and wide substrate specificity. It can preferentially decompose ester and peptide bonds adjacent to the C-terminus of hydrophobic, sulfur-containing, and aromatic amino acids, and is often used for protein degradation to produce short peptides. It has the typical catalytic triad Asp39 His69 Ser224 characteristic of serine proteases, and there are two Ca2+binding sites around the active center to increase its stability, allowing it to maintain high enzyme activity under a wider range of conditions.
Technical indicators:
Appearance: White to grayish white amorphous freeze-dried powder; Specific activity: ≥ 30 U/mg; No incision enzyme activity detected
Enzymatic properties:
Source: Tritirachiumalbum; Classification: EC 3.4.21.64; Molecular weight: 29 kDa (SDS-PAGE); Isoelectric point: 7.81; The optimal pH ranges from 7.0 to 12.0, indicating high activity; The optimal temperature is 65 ℃; PH stability pH 4.5-12.5 (25 ℃, 16 h); Thermal stability: Stable below 50 ℃ (pH 8.0,30 min), storage stability: Over 90% activity per year at 25 ℃, activator SDS, urea inhibitor diisopropylfluorophosphate (DFIP), benzoyl fluoride (PMSF); Storage conditions: Dry powder state -20 ℃, valid for 24 months; After the enzyme powder is dissolved, it should be divided into appropriate volumes for short-term storage at 2-8 ℃ and long-term storage at -20 ℃.
Purpose:
1. Gene diagnosis kit;
2. RNA and DNA extraction kit;
3. Extract non protein components from tissues and degrade protein impurities, such as the preparation of DNA vaccines and heparin;
4. Preparation of chromosome DNA for pulse electrophoresis;
5. Protein blotting;
6. Development and mass production of enzymatic glycosylated albumin reagents for in vitro diagnosis.
Precautions: Wear protective gloves and goggles when using or weighing, and store well ventilated after use. This product may cause skin allergic reactions. Inhalation may cause symptoms of allergies or asthma or difficulty breathing.
Activity measurement method: Enzyme activity definition: Unit enzyme activity is defined as the production of 1 protein per minute by hydrolyzing casein under specified conditions μ The amount of enzyme required for mol L-tyrosine.
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