SPECIFICATION
Product Description
The T7 High Yield RNA Transcription Kit (N1 Me Pseudo UTP) optimized the transcription reaction system, enabling users to efficiently obtain a large number of N1 Me Pseudo UTP modified RNA products through in vitro transcription reactions. The addition of modified nucleotides (N1 Me Pseudo UTP) can reduce the immune response of host cells. This kit uses T7 RNA polymerase to transcribe the downstream DNA sequence of the T7 promoter using linear double stranded DNA containing the T7 promoter sequence as a template and NTPs as a substrate to obtain single stranded RNA. This kit can also produce dyes, biotins, or radiolabeled RNA using different modified nucleotides as substrates; Produce capped RNA using hat structures or hat like structures as substrates.
This reagent kit has one reaction (20 μ L system) can obtain 150-200 μ G RNA products can be amplified to produce milligram level RNA. RNA synthesized by transcription can be used for applications such as RNA structure and function research, RNA enzyme protection, probe hybridization, RNAi, etc. It can also be capped by the cowpox virus capping system (Vazyme # DD4109) and 2 '- O-Methyltransferase (# DD4110), and E. coli Poly (A) Polymerase (# DD4111) tail added to produce mRNA for in vitro translation, transfection, microinjection, or other downstream applications.
