Solution for Disposable Plastic Consumables

Solution for Disposable Plastic Consumables

In the current field of life sciences, it is usually necessary for polymer materials to have hydrophilic, hydrophobic, temperature sensitive response and other characteristics on their surfaces to endow them with biocompatibility and adapt to the development needs of the biopharmaceutical industry. The commonly used surface modification technologies for polymer materials in the field of life sciences mainly include plasma treatment, plasma grafting polymerization.
In the current field of life sciences, it is usually necessary for polymer materials to have hydrophilic, hydrophobic, temperature sensitive response and other characteristics on their surfaces to endow them with biocompatibility and adapt to the development needs of the biopharmaceutical industry. The commonly used surface modification technologies for polymer materials in the field of life sciences mainly include plasma treatment, plasma grafting polymerization, and temperature sensitive material grafting polymerization modification technologies.

Plasma surface modification mainly involves two commonly used methods: plasma direct treatment and plasma grafting polymerization modification. The two methods have significant differences in stability, hydrophilicity, and degree of hydrophilicity when treating the surface of polymer materials to form a wetted surface.
1) The plasma direct treatment hydrophilic modification technology utilizes the plasma of non polymeric inorganic gases (Ar, N2, H2, O2, etc.) for surface reactions, introducing specific functional groups on the surface through surface reactions, resulting in surface erosion, forming cross-linked structural layers or generating surface free radicals.
2) Plasma grafting technology is a method of grafting another molecular material onto the surface of a material after non polymeric inorganic gas treatment, introducing - OH, - COOH, - NH2 on the surface of the material, thereby improving the hydrophilicity of the material surface. After plasma treatment with fluorinated gas, the surface of the material will undergo fluorination, which can be used to improve the surface hydrophobicity of the material.
Plasma treatment technology is used to initiate surface modification of polymer materials. The growth of cells must have a support surface that can be attached, relying on self secretion or adhesion factors provided in the culture medium to grow and proliferate on this surface, which requires the support surface to have hydrophilicity. The smaller the water contact angle, the better the cell adhesion and spreading, and the better the cell proliferation and differentiation.
Based on the application requirements of different consumables in biological laboratories, molecular structure design was carried out based on the structure and performance structure-activity relationship of polymer surface superhydrophobic, superhydrophilic, and temperature sensitive polymers. On the basis of systematic research on modification methods, surface structure and performance of modified products, a proprietary integrated technology was developed specifically for the subdivision field of disposable plastic consumables in biological laboratories.
The surface superhydrophilic modification technology of plasma induced grafting polymer materials has solved the hydrophilicity requirements of more cell cultures. In vitro cultivation of adherent cells requires the cultivation surface to have hydrophilicity. The company has hydrophilic modified the cultivation surface of the cell culture plate, bottle, and dish series products, with a hydrophilic contact angle of 30 to 40 °, which can meet the growth requirements of most cells; The neurogenic cells, stem cells, and primary cells in adherent cells require higher hydrophilicity on the surface of in vitro culture. The company's ultra hydrophilic cell culture surface preparation technology stabilizes the hydrophilicity contact angle of the culture surface below 10 °, meeting the needs of adherent culture of the aforementioned cells.


The surface superhydrophobic modification technology of plasma initiated graft polymer materials makes sample absorption and transfer more accurate. In molecular level life science research fields such as genomics and proteomics, such as PCR, RT PCR, qPCR, gene libraries, and protein chips, the accuracy requirements for reaction systems and sample transfer are very high. The hydrophobicity of ordinary pipettes is poor, and the residual rate is high during sample transfer, making it difficult to achieve accurate transfer. To meet this requirement, it is necessary for the surface of the suction head product to have stable superhydrophobic properties and high precision for micro pipetting. This technology enables the water contact angle to reach over 150 °, achieving low adsorption and residue on the material surface, and achieving a micro pipetting accuracy below the 2.2 ‰ level.
Compared with superhydrophobic suction heads, ordinary suction heads have significant liquid residues, forming a liquid film on the inner wall of the suction head; After surface treatment with superhydrophobic technology, the suction head has no obvious liquid residue, and the suction head is smooth and transparent without liquid film formation, almost achieving sample transfer without residue.
The temperature sensitive modification technology of polymer materials has achieved automatic cell detachment without damage. Compared with traditional cell harvesting methods such as enzymatic hydrolysis and mechanical methods, polymer material temperature sensitive modification technology achieves a change in the structure of polyisopropylacrylamide by lowering the temperature after cell culture, transforming its surface from superhydrophilic to superhydrophobic, and releasing and shedding cells without damage, with a shedding rate of over 90%.

The cell culture device uses 3D printing material modification technology to increase the number of cells harvested by 4-6 times. The key technology for preparing 3D cell culture scaffolds (3D printing material modification technology and 3D printing technology for cell culture devices) greatly improves the efficiency and yield of cell culture by increasing the cultivation area of the 3D cell culture scaffold by 4-5 times the corresponding 2D plane cell production, which is the mechanism of three-dimensional cell culture, intercellular interaction, cell immunotherapy, stem cell therapy And an ideal tool for drug screening research and cellular drug production.
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