2×PCR MasterMix
This product contains all the ingredients required for PCR, including Taq DNA polymerase, dNTPs, MgCl2, reaction buffer, PCR enhancer, electrophoresis tracer dye, etc. It has a wide range of uses.
Convenient, users only need to prepare templates and primers to conduct PCR experiments.
Product is 2 × The pre mixed solution and operating steps have been simplified to the maximum extent, which can reduce pollution and experimental errors.
Higher amplification efficiency and sensitivity.
After amplification, this product can be directly subjected to sample electrophoresis, further simplifying the operation.
The product can be directly used for T vector cloning without the need for additional A addition reaction.
This product is only for scientific research use.
Component 1mL × 10 1mL × 100 5mL × twenty
two × Taq PCR Mix (electrophoresis method) 1mL × 10 1mL × 100 5mL × twenty
1 instruction manual
Storage: -20 ℃, valid for one year.
At 30 μ Taking L's standard PCR reaction system as an example, add the following components to a clean PCR tube:
Ingredient dosage
two × Taq PCR Mix (electrophoresis method) 15 μ L
Template mammalian genomic DNA 0.5-1 μ G
Yeast Genomic DNA 5-500ng
Bacterial genomic DNA 0.5-50ng
Plasmid DNA 5-500pg
PCR recovery of fragments 1-100pg
PCR primer (self prepared) 10pmol each
DdH2O to 30 μ L
Place the PCR instrument for PCR, and take 5-10 samples after completion μ L direct sample electrophoresis (this product can be directly loaded without the need for sample solution), with a red tracer dye electrophoresis speed equivalent to 50bp DNA fragments.
Attention:
① If the reaction system is not 30 μ L. Each component needs to be increased or decreased proportionally.
② If there are unknown PCR inhibitors in the DNA template (which are commonly found in DNA samples such as plants, soil, and blood), a 1/10 PCR inhibitor scavenger (product number: BTN60804,30) can be added to the PCR system μ Add 3 in the L system μ L) It may be helpful for PCR.
