2×SuperSYBR PCR Mix(Low ROX)
This product is a premixed system specifically designed for real-time fluorescence quantitative PCR using the dye method (SYBR Green I), with a concentration of 2 ×， Contains BaldStar Taq DNA Polymerase, PCR Buffer, dNTPs, SYBR Green I fluorescent dyes, and Mg2+and Low ROX correction dyes, making the operation simple and convenient. Mainly used for detecting genomic DNA target sequences and cDNA target sequences after RNA reverse transcription.
The fluorescent dye SYBR Green I contained in this product can bind to all double stranded DNA, making it suitable for detecting different target sequences without the need to synthesize specific labeled probes. The BaldStar Taq DNA Polymerase contained in this product is a chemically modified, novel and highly efficient hot start enzyme that has no polymerase activity at room temperature, effectively avoiding non-specific amplification caused by non-specific binding of primers and templates or primer dimers at room temperature. The activation of the enzyme must be incubated at 95 ℃ for 10 minutes. The unique combination of PCR buffer system and hot start enzyme effectively inhibits non-specific PCR amplification, significantly improving the amplification efficiency of PCR.
The ROX dye contained in this product can correct the fluorescence signal error generated between the wells of the quantitative PCR instrument. The ROX correction dye content in this kit is relatively low, and is suitable for fluorescence quantitative PCR instruments such as ABI Prism7500/7500 Fast, Stratagene Mx3000/Mx3005P, Corbett Rotor Gene 3000 that require lower ROX signal correction.
This product uses a new and efficient hot start enzyme BaldStar Taq DNA Polymerase and a unique PCR buffer system, significantly improving the amplification efficiency of PCR, with high sensitivity and strong specificity.
Suitable for fluorescence quantitative PCR detection, capable of accurately quantifying and detecting target genes.
Component 5mL 40mL
two × SuperSYBR PCR Mix (Low ROX) 5 × 1mL 40 × 1mL
DdH2O 5 × 1mL 40 × 1mL
Storage: -20 ℃, avoid repeated freeze-thaw.
Before use, please gently mix it upside down and avoid foaming as much as possible. After briefly centrifuging, use it.
This product contains SYBR Green I fluorescent dye and ROX dye. When storing this product or preparing PCR reaction solution, strong light exposure should be avoided.
Avoid repeated freeze-thaw of this product, as repeated freeze-thaw may cause a decrease in product performance.
This product cannot be used for probe based fluorescence quantitative PCR.
When preparing the reaction solution, please use a new or non polluting gun head and centrifuge tube to prevent contamination as much as possible.
The following examples are the conventional PCR reaction system and reaction conditions. In practical operation, corresponding improvements and optimizations should be made based on different templates, primer structures, and target fragment sizes.
Preparation of PCR reaction system
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