5% BSA blocking buffer

5% BSA blocking buffer is suitable for blocking non-specific protein binding sites in WB, ELISA, and immunohistochemistry (IHC) experiments. After sealing, it can reduce the non-specific binding of subsequent primary or secondary antibodies with the carrier, reduce the background, enhance signal-to-noise ratio, and achieve ideal color rendering effect.
$27.00

SPECIFICATION

                                      5% BSA blocking buffer

5% BSA blocking buffer is suitable for blocking non-specific protein binding sites in WB, ELISA, and immunohistochemistry (IHC) experiments. After sealing, it can reduce the non-specific binding of subsequent primary or secondary antibodies with the carrier, reduce the background, enhance signal-to-noise ratio, and achieve ideal color rendering effect.
Saving conditions:
-Stored at 20 ℃, valid for 12 months
Usage:
This product is ready to use and does not require dilution. In the sealing step, add a sufficient amount of BSA sealing solution to fully cover the surface of the carrier, shake and seal. The sealing time is determined by the experiment, usually half an hour to one hour at room temperature, followed by subsequent operations such as antibody incubation.
Precautions:
Normally, it is sufficient to seal at room temperature for 60 minutes. For some antibodies with very high background, they can be sealed overnight at 4 ℃. For your safety and health, please wear laboratory clothes and disposable gloves for operation.
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