7.5% Sodium Chloride Broth for Selective Enrichment of Staphylococcus aureus
7.5% Sodium Chloride Broth is a selective enrichment medium for the cultivation of Staphylococcus aureus. The high-salt environment inhibits most unwanted microbes while allowing staphylococci to multiply.
Formula Composition (per Liter)
| Ingredient |
Content |
| Peptone |
10.0 g |
| Beef extract powder |
5.0 g |
| Sodium chloride |
75.0 g |
| Final pH |
7.4 ± 0.1 at 25 ℃ |
Testing Principle
Peptone and beef extract powder supply carbon, nitrogen, vitamins and mineral nutrients for bacterial growth. High concentration sodium chloride establishes high osmotic pressure, which inhibits the growth of most non-staphylococcal microorganisms.
Directions for Use
Weigh 90.0 g of the medium powder, heat and dissolve in 1000 mL distilled water, dispense into Erlenmeyer flasks or test tubes, autoclave at 121 ℃ for 15 minutes, and set aside.
Microbial Performance Test for 7.5% Sodium Chloride Broth
Prepare the medium according to the instructions and inoculate the designated quality control strains for incubation.
