Purpose: Used for selective isolation and cultivation of Pseudomonas aeruginosa.Composition (g/L)
Acetamide 10.0
Sodium chloride 5.0
Dipotassium hydrogen phosphate 1.39
Potassium dihydrogen phosphate 0.73
Magnesium sulfate 0.5
Phenol red 0.012
Agar 20.0
PH value 7.2 ± 0.1 25 ℃
Verification principle
Bacteria with acetamidase can utilize acetamide as a carbon source and decompose acetamide to produce alkali; Sodium chloride maintains a balanced osmotic pressure; Dipotassium hydrogen phosphate and potassium dihydrogen phosphate are used as buffering agents; Magnesium sulfate provides the necessary ions for growth; Agar is a coagulant for the culture medium; Phenol red is a pH indicator.
Usage: Weigh 37.6g of this product, heat and dissolve it in 1000ml of distilled water, pack separately, sterilize under high pressure at 121 ℃ for 15 minutes, set aside.
Note: The culture medium contains magnesium sulfate and acetamide, and there is a small amount of precipitation after sterilization.
Acetamide agar microbial sensitivity test:
Prepare the culture medium according to the label usage, inoculate the quality control strain, and incubate it aerobic at 36 ± 1 ℃ for 24 ± 2 hours.
