The constant temperature color change amplification kit includes: HNB color change amplification kit, orange green color change amplification kit, and turbidity amplification kit. Among them, hydroxynaphthol blue (HNB) is a metal ion indicator. The combination of HNB and magnesium ions causes the initial color of the reaction system to be violet. As the reaction progresses, Mg2+reacts with the precipitated pyrophosphate ions to form magnesium pyrophosphate precipitate. The loss of magnesium ions in hydroxynaphthol blue causes the system color to become sky blue, while the unreacted system still maintains violet color; The orange green color changing dye (OG Dye) can combine with LAMP amplification products to form a visible fluorescent green color, while the negative reaction is orange; The turbidity rule is used to determine the reaction result by detecting or visually observing the precipitate of magnesium pyrophosphate produced by LAMP intense reaction using a turbidity meter. Our company has launched the African swine fever detection kit based on visual constant temperature amplification, which is a freeze-dried product. Compared to liquid products on the market, it is more stable, sensitive, and easy to operate. The detection is usually completed within 30 minutes.
Main features
1. The single tube dual design makes the detection data more reliable and avoids false negatives caused by sample errors.
2. The maximum sensitivity is 50 viruses/swabs; Sensitivity is at the single copy level.
3. Use a specific double labeled probe with 100% accuracy and no false positives.
4. Complete the entire process of closed tube inspection within 25 minutes; Each detection tube contains more than 100 virus samples, and the detection can be completed within 10 minutes.
5. Nucleic acid is free of extraction and equipped with virus lysis solution. The vortex can complete the lysis in 10 seconds.
6. Equipped with portable constant temperature fluorescence detection equipment, it can be operated in the wild, communities, homes, workplaces, and any environment.
7. Easy to operate, easy to learn, easy to understand, without the need for complex training work
Reagent components
NAME |
100T×20μl |
Lyophilized DNA HNB PCR Reagent |
1 bottle |
2×TaqMan PCR Buffer5 |
1ml |
Storage
1. Lyophilized ASFV TaqMan PCR Reagent is in the form of freeze-dried products, containing hot start Fast Taq DNA polymerase, dNTP, amplification primers, and probes. Each bottle can be used for 50 amplification reactions.
2. Lyophilized ASFV TaqMan PCR Reagent can be stored at room temperature for 6 months, 4 ℃ for 12 months, -20 ℃ for long-term storage, and can be transported at room temperature.
3. Freeze dried products undergo 1 × After TaqMan PCR Buffer2 is dissolved, it can be stored for a long time at -20 ℃.
4.1 × TaqMan PCR Buffer2 can be stored at room temperature for a long time, but is usually stored at 4 ℃ or -20 ℃.
Reaction examples