• African Swine Fever Real-time Fluorescence PCR Detection Kit(Lyophilized)
  • African Swine Fever Real-time Fluorescence PCR Detection Kit(Lyophilized)
  • African Swine Fever Real-time Fluorescence PCR Detection Kit(Lyophilized)
  • African Swine Fever Real-time Fluorescence PCR Detection Kit(Lyophilized)

African Swine Fever LAMP-TaqMan Detection Kit(Lyophilized)

1. The single tube dual design makes the detection data more reliable and avoids false negatives caused by sample errors. 2. The maximum sensitivity is 50 viruses/swabs; Sensitivity is at the single copy level. 3. Use a specific double labeled TaqMan probe with 100% accuracy and no false positives. 4. Complete the entire process of closed tube inspection within 25 minutes; Each detection tube contains more than 100 virus samples, and the detection can be completed within 10 minutes.
$5.50
  • African Swine Fever Real-time Fluorescence PCR Detection Kit(Lyophilized)
  • African Swine Fever Real-time Fluorescence PCR Detection Kit(Lyophilized)

SPECIFICATION

Product Introduction
Using LAMP (Loop mediated isothermal amplification) amplification technology, under constant temperature conditions, the characteristics of nucleic acid molecules are amplified exponentially, amplifying RNA or DNA 109-1010 times within 25 minutes. Real time detection using FAM-BHQ fluorescence cutting probe can be performed on conventional quantitative PCR instruments or portable constant temperature fluorescence detectors. Multiple LAMP (Multi Plex LAMP) loop amplification technology was used in conjunction with specific double labeled fluorescent probes for the detection of African swine fever virus. The dual African swine fever constant temperature fluorescent nucleic acid detection kit developed by our company adopts a single tube dual design, with the target gene being the P72 gene of African swine fever virus and the beta actin gene of pig internal reference; In the case of direct use of swab soaking solution, no nucleic acid extraction is required, and the detection is completed within 25 minutes. The effective sensitivity of this kit is 200 viruses/swab.

Main features
1. The single tube dual design makes the detection data more reliable and avoids false negatives caused by sample errors.
2. The maximum sensitivity is 50 viruses/swabs; Sensitivity is at the single copy level.
3. Use a specific double labeled TaqMan probe with 100% accuracy and no false positives.
4. Complete the entire process of closed tube inspection within 25 minutes; Each detection tube contains more than 100 virus samples, and the detection can be completed within 10 minutes.
5. Nucleic acid is free of extraction and equipped with virus lysis solution. The vortex can complete the lysis in 10 seconds.
6. Equipped with portable constant temperature fluorescence detection equipment, it can be operated in the wild, communities, homes, workplaces, and any environment.
7. Easy to operate, easy to learn, easy to understand, without the need for complex training work

Reagent components
NAME 100T×20μl
Lyophilized DNA TaqMan PCR Reagent 1 bottle
2×TaqMan PCR Buffer5 1ml 

Storage
1. Lyophilized ASFV TaqMan PCR Reagent is in the form of freeze-dried products, containing hot start Fast Taq DNA polymerase, dNTP, amplification primers, and probes. Each bottle can be used for 50 amplification reactions.
2. Lyophilized ASFV TaqMan PCR Reagent can be stored at room temperature for 6 months, 4 ℃ for 12 months, -20 ℃ for long-term storage, and can be transported at room temperature.
3. Freeze dried products undergo 1 × After TaqMan PCR Buffer2 is dissolved, it can be stored for a long time at -20 ℃.
4.1 × TaqMan PCR Buffer2 can be stored at room temperature for a long time, but is usually stored at 4 ℃ or -20 ℃.

Reaction examples
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