This product is used for sample closure in immune labeling experiments. Blocking serum can block non specific protein binding sites and endogenous Fc fragment binding sites in the sample, blocking antibody binding to Fc receptors in the sample, thereby reducing background and false positives. When selecting a blocking serum, it is important to note that the blocking serum should not contain the target protein and should not be from the same source as the primary antibody. It can be used for serum from the same source as the secondary antibody.
This product is a sealed donkey serum stock solution, collected aseptically, filtered three times at 0.1 um, and then packaged separately.
Saving conditions:
-Stored at 20 ℃, valid for 5 years
Usage:
When using, dilute the sealed serum stock solution 10 or 20 times with PBS (0.01mol/LpH7.2-7.4), prepare it into a 10% or 5% working solution, and directly drip it onto tissue slices or cell smears. Incubate at 37 ℃ for 10-30 minutes to remove the serum, do not wash it, and drip an appropriately diluted primary antibody working solution. Incubate at 37 ℃ for 1-2 hours or 4 ℃ overnight.
Precautions:
1. Before use, it is necessary to thaw at 4 ℃ and dissolve evenly before use. It is recommended to divide it into small portions and store at -20 ℃. 2. The diluted serum should not be stored for too long and should be used as soon as possible to avoid bacterial contamination. Suggest using it as needed.
3. This product is limited to scientific research by professionals and shall not be used for clinical diagnosis or treatment, nor for food or medicine.
For your safety and health, please wear laboratory clothes and disposable gloves for operation.