This product, BsaI, is a protein mutant encoded by the BsaI gene of Bacillus stearothermophilus expressed in recombinant E. coli. It is an IIS class restriction endonuclease,
The recognition sequence and cleavage sites are as follows:
5'······GGTCTC(N)···············3'
3'······CCAGAG(NNNNN)······5'
