Cell Counting Kit-8 (CCK-8)
Cell Counting Kit-8 (CCK-8) is a rapid and highly sensitive detection kit widely used for cell proliferation and cytotoxicity based on WST-8.
The CCK-8 reagent contains WST-8 (chemical name: 2- (2-methoxy-4-nitrophenyl) -3- (4-nitrophenyl) -5- (2,4-disulfonobenzene) -2H-tetrazole monosodium salt), which is a compound similar to MTT. In the presence of the electron coupling reagent 1-methoxy-5-methylphenazionium dimethyl sulfate (1-Methoxy PMS), it can be reduced by dehydrogenase in mitochondria to a highly water-soluble orange colored formazan product, The number of Formazan generated is proportional to the number of live cells. Therefore, this characteristic can be directly used for cell proliferation and toxicity analysis. The more cells proliferate, the faster, and the darker the color; The greater the cytotoxicity, the lighter the color.
Applicable scope:
It can be widely used in drug screening, cell proliferation testing, cytotoxicity testing, and tumor drug sensitivity testing.
Product advantages:
The CCK-8 reagent kit has the characteristics of high sensitivity, short reaction time, wide linear range, reliable data, and good reproducibility.
Saving method:
Store at 4 ℃ away from light, valid for one year- 20 ℃ can be stored for a longer time, but repeated freeze-thaw can increase the background value and interfere with experimental measurements. Therefore, please store frequently used reagents at 4 ℃.