Purpose: Used for selective isolation and cultivation of Pseudomonas aeruginosa.
Composition (g/L)
Peptone 10.0
Sodium chloride 5.0
Beef Flour 3.0
Cetane trimethylammonium bromide 0.3
Agar 20.0
PH value 7.5 ± 0.1 25 ℃
Effect
1. Nutrient composition: Pseudomonas aeruginosa has a strong ability to decompose proteins, while its ability to ferment sugars is low. In the medium components, gelatin trypsin hydrolysate and glycerol provide carbon, nitrogen, vitamins, and growth factors for the growth of Pseudomonas aeruginosa.
2. Inorganic salts: Magnesium chloride and potassium sulfate can promote the production of pyocyanin and maintain osmotic pressure.
3. Principle of selective cultivation: Cetrimide, as a quaternary ammonium salt cationic surfactant, changes the permeability of bacterial cells, causing bacterial autolysis or denaturation and precipitation of bacterial proteins, leading to bacterial death, and has a strong inhibitory effect. Pseudomonas aeruginosa has a certain tolerance to cetyltrimethylammonium bromide, therefore, it is used for selective cultivation of Pseudomonas aeruginosa.
4. Color: During the growth process of Pseudomonas aeruginosa, two water-soluble pigments are produced: yellow fluorescein and green pyocyanin, so the colony appears yellow green on the cetyltrimethylammonium bromide agar plate.
Usage:
Weigh 38.3g of this product, boil it and dissolve it in 1000ml of distilled water. Divide it into triangular bottles and sterilize under high pressure at 121 ℃ for 15 minutes. Set aside.
Cetane trimethylammonium bromide agar microbial sensitivity test:
Prepare the culture medium according to the label usage, inoculate the following quality control strains, and incubate them under aerobic conditions at 36 ± 1 ℃ for 18-24 hours.
