Cell free DNA storage tubes at room temperature can effectively stabilize the free DNA (cfDNA) in blood samples, prevent the release of genomic nucleic acid from non targeted background nucleated blood cells, and inhibit the degradation of free nucleic acid by nucleases, thereby ensuring the stability of cfRNA in plasma samples. The separated free nucleic acid is suitable for downstream PCR, high-throughput sequencing, and other gene detection fields.
Ordering information: 10mL, 50 pics per box
Product advantages:
1、 Compliant and reliable● Fully self produced
● Sterile supply
● Exclusive patent protection
Registration License for Class II Medical Devices
2、 High quality and stabilityPreserve cell free DNA and cell free RNA in whole blood at room temperature for 7 days, suitable for gene detection fields such as tumor liquid biopsy and unknown infections
Reduce repeated blood draws for patients, dual use with one tube
Stable negative pressure and dedicated blood collection vessels suitable for use in high altitude areas
High quality glass material, greatly reducing the risk of pipe breakage and cracking
Valid for up to 18 months
3、 Professional and meticulous after-sales serviceUnder extreme external transportation temperature conditions (below 4 ℃ and above 37 ℃), supporting solutions can be provided
R&D products can be customized with personalized outer packaging, providing a highly competitive price advantage.
Product validation data 1.Agilent 2100 detection of cf-DNA and cf-RNAAgilent 2100 Bioanalyzer detects the concentration and integrity of free nucleic acids (cell free DNA and cell free RNA). Collect peripheral blood samples using Beyondbio , Street, and EDTA tubes and store them at room temperature for 7 days. Separate 1mL of plasma to extract cell free DNA and cell free RNA, respectively. Agilent 2100 test results are visible
Figure 1. When stored at room temperature until the 7th day, the cell free DNA sample concentration and integrity of the Beyondbio preservation tube were stable.
Figure 2. When the plasma cell free RNA samples in EDTA tubes were stored for 3 days, multiple fragments of varying sizes appeared and the concentration also significantly increased. The concentration in Street tubes was lower than that in Beyondbio tubes; At 7 days, the concentration and integrity of the Beyondbio preservation tube cell free RNA sample were still well preserved.
2、 Digital PCR detection of cf-DNA and cf-RNADroplet digital PCR was used to detect the copy number changes of c-fos gene in Beyondbio preservation tubes in their plasma cf-DNA and cf-RNA samples.
Figure 3. C-fos gene copy number results of cf-DNA in Lakebio preservation tube plasma samples. It can be seen that after being stored at room temperature for 7 days, there was no significant fluctuation in the c-fos gene content of cf-DNA samples detected by digital PCR.
Figure 4. The c-fos gene copy number results of plasma samples cf-RNA stored in Beyondbio storage tubes on days 0, 3, and 7 were compared with those in Street and EDTA tubes. The results showed that the c-fos gene quantity of cf-RNA samples stored in Lakebio storage tubes at room temperature for 7 days was very stable.
3.Comparison of Hemolysis Status between Beyondboi and Streck Blood Collection Vessels
Figure 1: Beyondboi's blood collection tube and Streck's blood collection tube collect the same sample. After being stored at room temperature for 7 days, the plasma is separated by centrifugation at 1600g for 10 minutes
Figure 2 :shows the EDTA blood collection tube of the same patient. It can be seen that after 7 days of transportation, the Beyondbo blood collection tube sample still shows clear upper layer plasma without hemolysis, and the upper layer plasma volume is similar. After being stored at room temperature for 14 days, EDTA blood collection tubes showed significant rupture of red blood cells and white blood cells, while Beyond bio blood collection tubes had intact blood cell morphology.
