Product Name:
2-Chloro-4-nitrobenzene- α- L-fucoside; Abbreviation: CNP-AFU; CAS: 157843-41-9; Appearance: White powder; Molecular formula/molecular weight: C12H14ClNO7/319.70; Purity: ≥ 99%; Detection wavelength: λ Max (color rendering wavelength)=405 nm; Storage conditions: 2-8 ℃, sealed, dry, and protected from light. Long term recommended; Store in dark at -25~-15 ℃, shelf life: 2 years
Purpose:
Used to detect the content of fucosidase (AFU) in serum, and to develop and extensively prepare AFU detection reagents. Reaction principle: AFU in serum acts on the substrate to generate 2-chloro-4-nitrobenzene (CNP), and the absorbance change rate of CNP per minute is measured at a wavelength of 405nm（ Δ A/min), calculate its activity.
Performance evaluation:
Based on the characteristics of kinetic reactions, the optimal reaction conditions such as ion strength, pH value, substrate concentration, and various experimental parameters of the buffer solution were studied. Perform corresponding performance analysis and research on the AU400 fully automatic biochemical analyzer using this method. The minimum detection limit of this method was 2.5U/L, with a linear range of 250U/L (r=0.9994). Intra batch CV3%, inter batch CV5%, and recovery of 101.3%. Conclusion: This method has high sensitivity, good linearity, precision, accurate results, and is easy and fast to operate. It is suitable for the operation of fully automatic biochemical analyzers.
Advantages:
1) Good water solubility;
2) High sensitivity;
3) Good linearity;
4) Good stability
matters needing attention
Materials used in in vitro diagnostic products shall not be used in human or in vivo experiments.
After taking it out of the refrigerator, it should be placed at room temperature to ensure a balanced temperature difference between the inside and outside of the bottle before opening the lid for use. Avoid directly opening the lid to absorb moisture and store it in a dry and dark place
Purpose: Immunoassay using alkaline phosphatase (ALP) as a marker.
