Direct One Tube RT-PCR
One tube RT-PCR is different from the traditional two-step method (reverse transcription and PCR amplification are completed in two steps). RNA template reverse transcription and cDNA amplification are completed in one tube, greatly reducing the risk of contamination, shortening analysis time, and improving the repeatability of the reaction. One Tube RT-PCR uses an optimized buffer system, high purity enzymes, and no DNA or RNA residues. High activity M-MLV reverse transcriptase (RNase H -) is used as the reverse transcriptase, and hot start high fidelity KOD enzyme is used for PCR amplification, significantly reducing mismatch.
RT-PCR products can be analyzed by agarose gel electrophoresis and EB staining, as well as downstream routine operations, such as restriction endonuclease digestion, ligation, DNA sequencing, dHPLC analysis; Alternatively, a quantitative PCR instrument can be used for qRT PCR for online analysis.
Product application:
• cDNA cloning (flat end connection, restriction enzyme digestion, agarose gel electrophoresis/EB staining, fluorescent capillary electrophoresis, DNA sequencing, etc.);
• Especially suitable for qRT PCR analysis of RNA;
Quantitative or semi quantitative analysis of RNA viruses or RNA samples.
Product advantages:
(1) No need for separate management, simple and convenient, reducing pollution risk, and shortening analysis time;
(2) The enzymes and other reagents required for the reaction outside of the buffer are pre mixed Mixes, ensuring accurate dosage and convenient operation;
(3) High activity and stability of reverse transcriptase and DNA polymerase ensure high sensitivity and specificity of the reaction;
(4) DNA polymerase uses hot start KOD, which has a fidelity higher than Taq enzyme by more than 20 times (the product is suitable for flat end connection);
(5) Especially suitable for quantitative or semi quantitative analysis of RNA samples.
Storage conditions: One Tube RT-PCR kits can be stored at -20 ℃ and have a validity period of more than 1 year.
