• CHO DNA assay Kit
  • CHO DNA assay Kit

DNase assay Kit(Fluorescence)

1) Used for quantitative detection of CHO host cell DNA residues in intermediate, semi-finished, and finished products of various biological products. (2) Fast detection, strong specificity, reliable performance, and the minimum detection limit can reach the fg level. (3) The CHO DNA quantitative reference material matched with the reagent kit has been traced back to the national standard.
$199.00
  • CHO DNA assay Kit

SPECIFICATION

Specification: 48T (freeze-dried probe).
Intended use: This reagent kit is used to detect the contamination status of DNase in samples.
Testing principle: The DNase detection kit is based on a DNA probe labeled with fluorescent groups. When the sample does not contain DNase activity, the probe remains stable and does not generate fluorescence signals; When the sample contains DNase activity, the probe is degraded, producing a gradually enhanced fluorescence signal; The rate of increase in fluorescence signal is positively correlated with the number and activity of enzymes. Measure the fluorescence signal increase factor using a fluorescence enzyme-linked immunosorbent assay at ex/em=485/525nm wavelength to determine whether the sample is contaminated with DNase.

Storage conditions and expiration date
1. The unopened reagent kit is stored at -20 ℃ for 12 months.
2. After opening, store at -20 ℃ for 6 months. It is recommended to pack the diluted DNA probe working solution according to the single use amount to avoid repeated freeze-thaw.
Required equipment and consumables: Fluorescence enzyme-linked immunosorbent assay (including ex/em=485/525nm wavelength); No DNase, no RNase pipette and suction head; No DNase, no RNase EP pipe; No DNase No RNase Black 96 well Plate

Preparation before experiment
1. Take out the reagent kit and balance it to room temperature (18-25 ℃), 10 × Reaction solution, TE buffer, DNase I standard (2U/ μ L) Fully shake and mix the standard dilution and other components, and then centrifuge them instantaneously (4000-7000rpm for 10 seconds).
2. Centrifuge the DNA probe at 4000-7000rpm for 60 seconds to gather at the bottom of the tube, carefully open the tube cap, and dilute it 50 times with TE buffer (12 μ L DNA Probe Addition 588 μ L TE buffer solution, used as a working solution for DNA probes. The unused DNA probe working solution should be packaged according to the individual usage and stored at -20 ℃ to avoid repeated freeze-thaw cycles
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