Specification: 48T (freeze-dried probe).
Intended use: This reagent kit is used to detect the contamination status of DNase in samples.
Testing principle: The DNase detection kit is based on a DNA probe labeled with fluorescent groups. When the sample does not contain DNase activity, the probe remains stable and does not generate fluorescence signals; When the sample contains DNase activity, the probe is degraded, producing a gradually enhanced fluorescence signal; The rate of increase in fluorescence signal is positively correlated with the number and activity of enzymes. Measure the fluorescence signal increase factor using a fluorescence enzyme-linked immunosorbent assay at ex/em=485/525nm wavelength to determine whether the sample is contaminated with DNase.
Storage conditions and expiration date
1. The unopened reagent kit is stored at -20 ℃ for 12 months.
2. After opening, store at -20 ℃ for 6 months. It is recommended to pack the diluted DNA probe working solution according to the single use amount to avoid repeated freeze-thaw.
Required equipment and consumables: Fluorescence enzyme-linked immunosorbent assay (including ex/em=485/525nm wavelength); No DNase, no RNase pipette and suction head; No DNase, no RNase EP pipe; No DNase No RNase Black 96 well Plate
Preparation before experiment
1. Take out the reagent kit and balance it to room temperature (18-25 ℃), 10 × Reaction solution, TE buffer, DNase I standard (2U/ μ L) Fully shake and mix the standard dilution and other components, and then centrifuge them instantaneously (4000-7000rpm for 10 seconds).
2. Centrifuge the DNA probe at 4000-7000rpm for 60 seconds to gather at the bottom of the tube, carefully open the tube cap, and dilute it 50 times with TE buffer (12 μ L DNA Probe Addition 588 μ L TE buffer solution, used as a working solution for DNA probes. The unused DNA probe working solution should be packaged according to the individual usage and stored at -20 ℃ to avoid repeated freeze-thaw cycles