• dNTP Solution
  • dNTP Solution

dNTP Solution

This product is a mixture of dATP, dTTP, dGTP, and dCTP nucleotides, with a concentration of 10mM for each nucleotide and a purity of over 98% (HPLC detection)
$15.00
  • dNTP Solution

SPECIFICATION

               dNTP Solution
This product is a mixture of dATP, dTTP, dGTP, and dCTP nucleotides, with a concentration of 10mM for each nucleotide and a purity of over 98% (HPLC detection). It does not contain DNA endonuclease, DNA exonuclease, RNA enzyme, and phosphatase contamination and can be directly used for PCR, RT-PCR, cDNA synthesis, DNA sequencing, DNA probe labeling, and other reactions. The concentration used depends on the specific reaction, please refer to the relevant manual.
Molecular structure of dNTP
Storage conditions: low-temperature transportation and storage at -20 ℃, with a validity period of one year.
Why does DNA use 2 '- deoxyribose and thymine?
Why doesn't DNA choose ready-made photosynthesis products such as ribose and the raw material for synthesizing RNA, uracil, as its sugar molecules and bases, instead of selecting 2 '- deoxyribose and thymine, which require additional synthesis steps to obtain? What is the reason for this? The current mainstream view is that one of the reasons why DNA cannot choose ribose is because the OH group at the 2 'position of ribose will result in an uneven and non parallel double helix skeleton structure, making it difficult to effectively fold into the chromosome structure. However, using 2' - deoxyribose can result in a uniform and parallel double helix structure; The second reason is that the OH group at the 2 'position of the ribose will carry out intramolecular nucleophilic attacks on adjacent phosphate diester bonds, reducing molecular stability. Stability is crucial for genetic material, so DNA chose 2' - deoxyribose that has lost its intramolecular nucleophilic attack ability. For messenger molecules, stability is not important, so RNA still uses ribose.
The reason for using thymine in DNA is that cytosine in DNA slowly hydrolyzes into uracil. If uracil is also a normal component of DNA, the DNA repair system cannot distinguish which uracil is normal and which needs to be repaired. The use of thymine (which has one more methyl group than uracil molecules) not only maintains the same ability to form base pairs as uracil, but also distinguishes it from uracil, allowing any uracil present in DNA molecules to be discovered and repaired by uracil DNA glycosylase
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