• Laurye Sulfate Tryptose Broth With MUG
  • Laurye Sulfate Tryptose Broth With MUG

Eosin Methylene Blue Agar

Eosin Methylene Blue Agar (EMB Agar) is a selective differential medium for isolating Gram-negative enteric bacteria, especially coliforms and fecal coliforms. Eosin and methylene blue inhibit Gram-positive bacteria and act as pH indicators; lactose-fermenting strains form purple-black colonies or black-centered colonies with transparent edges.
$2.00
  • Laurye Sulfate Tryptose Broth With MUG

SPECIFICATION

Eosin Methylene Blue Agar (EMB Agar)

Eosin Methylene Blue Agar (EMB Agar) is widely used for the isolation of Gram-negative enteric bacteria, particularly coliform and fecal coliform groups. It combines selective inhibition and differential identification based on lactose fermentation.

Inspection Principle

Peptone supplies carbon and nitrogen sources for bacterial growth. Lactose serves as fermentable carbohydrate for E. coli group. Dipotassium hydrogen phosphate works as buffering agent. Agar is the gelling agent. Eosin and methylene blue function as both bacteriostatic agents and pH indicators. They suppress Gram-positive bacteria and form precipitates under acidic conditions. Lactose fermenters produce purple-black colonies or colonies with black centers and colorless transparent peripheries.

Directions for Use

Take one sachet of the product, add into 200 mL distilled or deionized water, stir and heat to boiling until fully dissolved. Autoclave at 121 ℃ for 15 minutes. Cool the medium to approximately 50 ℃, pour into sterile plates under aseptic conditions, allow to solidify and reserve for use.
CONTACT US
Name *
Email *
Message *
Verification code *
Click to verify