GC-rich PCR Buffer
This kit supplies four different GC-rich PCR buffers to offer multiple options for amplifying high GC-content DNA fragments and significantly improve amplification success rates. It is compatible with widely used thermostable DNA polymerases such as Taq DNA polymerase and Pfu DNA polymerase.
The four GC-rich PCR buffers can be used either alone or in combination to optimize PCR performance across diverse templates. Recommended optimized combinations are available for direct selection.
As documented in literature, a single GC-rich buffer may work well for one high-GC fragment but fail for another. No universal GC buffer suits all high-GC templates, making high-GC amplification a long-standing challenge in molecular biology. To address this issue, this 4-tube GC-rich PCR Buffer kit provides multiple conditions for flexible optimization and combination.
Component Specifications
5× GC rich PCR Buffer A: 0.5 mL
5× GC rich PCR Buffer B: 0.5 mL
5× GC rich PCR Buffer C: 0.5 mL
5× GC rich PCR Buffer D: 0.5 mL
Storage
Important Notes
When using buffers from this kit, a polymerase-compatible PCR buffer containing magnesium ions (final 1×) must be used together.
PCR is highly sensitive and can amplify target sequences over 10 million-fold. Strictly avoid trace template DNA contamination during setup. Blank no-template controls are recommended to check for contamination.
PCR thermal cycling parameters may follow the manual of your selected thermostable polymerase. For high-GC templates, extension time is often longer than standard protocols.
Reaction system setup follows the polymerase manual; only add an appropriate volume of the GC-rich buffer from this kit. A reference system is shown below:
| Component |
Final Conc. |
50 μL System |
20 μL System |
| ddH₂O / Milli-Q Water |
— |
(26.75−x) μL |
(10.7−y) μL |
| 10× PCR Buffer (with Mg²⁺) |
1× |
— |
— |
