GC-rich PCR Buffer
This product provides four different GC rich PCR Buffers, providing various options for expanding DNA fragments with increased GC content, greatly improving the success rate of amplification of high GC content DNA fragments. It can be applied to various common heat-resistant DNA polymerase such as Taq DNA polymerase, Pfu DNA polymerase, etc.
Various GC rich PCR buffers can be used alone or in combination. This can optimize PCR conditions under more conditions. Recommended combinations of optimized GC Rich PCR Buffers are provided for users to choose from directly.
According to relevant literature reports, a single GC rich PCR buffer has a good amplification effect on a high GC content DNA segment, but it is likely that the amplification effect is not ideal for another high GC content DNA segment. Because it is difficult to use a universal GC rich PCR buffer to amplify different high GC content DNA fragments, the amplification of high GC content DNA fragments has always been a challenge in molecular biology operations. In response to this practical situation, Biotech has launched a 4-tube GC Rich PCR Buffer that can provide users with multiple conditions for optimization and combination.
Component specifications
five × GC rich PCR Buffer A 0.5mL
five × GC rich PCR Buffer B 0.5mL
five × GC rich PCR Buffer C 0.5mL
five × GC rich PCR Buffer D 0.5mL
Storage: -20 ℃
When using the GC rich PCR buffer provided by this kit, it is necessary to simultaneously use a magnesium ion containing PCR buffer with a final concentration of 1X that is compatible with DNA polymerase.
Due to the highly sensitive PCR reaction, which can amplify the target gene sequence by more than 10 million times, please pay attention to avoiding contamination of trace amounts of DNA to be amplified during operation, and try to set up blank controls without templates to confirm whether there is contamination of DNA to be amplified.
The setting of PCR reaction parameters can also refer to the instructions for specific heat-resistant DNA polymerase. Usually, for the amplification of high GC content DNA fragments, the extension time can be appropriately extended compared to the conventional extension time.
The setting of the PCR reaction system can refer to specific heat-resistant DNA polymerase, and only an appropriate amount of GC rich PCR buffer provided by this kit can be added. You can refer to the following reaction system to set up a PCR reaction:
Final concentration of ingredients 50 μ L system 20 μ L-system
Double steamed water or Milli Q water - (26.75 x) μ L (10.7-y) μ L
ten × PCR Buffer (wit