• Human Liver Ductal Organoid Kit (Differentiation)
  • Human Liver Ductal Organoid Kit (Differentiation)
  • Human Liver Ductal Organoid Kit (Differentiation)
  • Human Liver Ductal Organoid Kit (Differentiation)

Human Liver Ductal Organoid Kit (Differentiation)

Organoids refer to tissue analogues with a certain spatial structure formed by three-dimensional cultivation of adult stem cells or pluripotent stem cells in vitro. Organs are highly consistent with their source tissues in terms of tissue structure, cell type, self-renewal ability, and function, thus demonstrating unique advantages in multiple biomedical fields such as developmental biology, disease modeling, precision medicine, drug research and development, gene and cell therapy, infection and immunity, and regenerative medicine.
  • Human Liver Ductal Organoid Kit (Differentiation)
  • Human Liver Ductal Organoid Kit (Differentiation)

SPECIFICATION

Organoids refer to tissue analogues with a certain spatial structure formed by three-dimensional cultivation of adult stem cells or pluripotent stem cells in vitro. Organs are highly consistent with their source tissues in terms of tissue structure, cell type, self-renewal ability, and function, thus demonstrating unique advantages in multiple biomedical fields such as developmental biology, disease modeling, precision medicine, drug research and development, gene and cell therapy, infection and immunity, and regenerative medicine.

Product Description:

Human Liver Ductal Organoid Kit is a chemically defined cell culture medium for establishment and maintenance of human liver ductal organoids(hLDs) derived from adult stem cells. Self-renewal of the liver ductal epithelium is driven by the proliferation of stem cells and their progenitors located in liver. hLDs display all hallmarks of the liver ductal epithelium in terms of architecture, cell type composition, and self-renewal dynamics, therefore hold great promise for unprecedented studies of Human liver development and disease, hLDs could differentiate into hepatocyte like cell under the induction of differentiation medium.

Product instructions
Use sterile technique to prepare the human liver ductal organoid differentiation medium. hLDs grown in Human Liver Ductal Organoid Expansion Medium overwhelmingly consisted of cholangiocytes. After changing the Expansion Medium to differentiation medium, the hLDs could differentiate into hepatocyte like cells, which display the markers of hepatocytes, including ALBUMINTTR and CYP3A4. The following examples are for preparing 10 mL of Differentiation I Medium and Differentiation II Medium. If preparing other volumes, adjust accordingly.
1.  Thaw Human Liver Ductal Organoid Supplement B(50x) (Differentiation), Human Liver Ductal Organoid Supplement C(250x) (Differentiation) and Human Liver Ductal Organoid Supplement D(250x) (Differentiation) on ice.
NOTE: Once thawed, use immediately or aliquot and store at -20°C for not more than 10 months. After thawing the aliquots, use immediately. Do not re-freeze.
2.  For Human Liver Ductal Organoid Differentiation Medium I. Add 40 μL Human Liver Ductal Organoid Supplement D(250x) (Differentiation) to 10 mL Human Liver Ductal Organoid Medium(Expansion) (K2008-HLD). Mix thoroughly.
3.  For Human Liver Ductal Organoid Differentiation Medium II. Add 200 μL Human Liver Ductal Organoid Supplement B(50x) (Differentiation) and 40 μL Human Liver Ductal Organoid Supplement C(250x)(Differentiation)to 10 mL Human Liver Ductal Organoid Basal Medium (Differentiation). Mix thoroughly.
NOTE: 
If not use immediately, store complete medium at 2-8°C for not more than 2 weeks. bioGenousTM Human Liver Ductal Organoid Supplement B (Differentiation) contains fungicide and antibiotics(50x).

Product Information:

Component

Item NO.

Volume

Storage& Stability

 Human Liver Ductal Organoid Basal Medium(Differentiation)

K2008-HLH –A100/A500

100mL/500mL

4℃,12 months

Human Liver Ductal Organoid Supplement B(50x)(Differentiation)

K2008-HLH –B100/B500

2mL/10mL

-20℃,avoid repeated freeze-thaw cycles, 12 months

Human Liver Ductal Organoid Supplement C(250x) (Differentiation)

K2008-HLH –C100/C500

0.4mL/2mL

-20℃, avoid repeated freeze-thaw cycles, 12 months

Human Liver Ductal Organoid Supplement D(250x) (Differentiation)

K2008-HLH –D100/D500

0.4mL/2mL

-20℃, avoid repeated freeze-thaw cycles, 12 months

 

 

CONTACT US
FirstName*
LastName*
Email*
Message*