Hypersensitive ECL Chemiluminescence Kit
This product is a chemiluminescence reagent kit based on luminol substrate, which can be catalyzed by horseradish peroxidase (HRP) and emit strong glow, with a detection sensitivity of fg level. This reagent kit has optimized the substrate composition and used a new high-efficiency enhancer, which increases the luminescence intensity by 100-1000 times compared to traditional ECL colorants and effectively reduces the background. The reagent kit uses new oxidants to replace unstable hydrogen peroxide, which improves the stability of the kit and can be stably stored at room temperature for one year. After being catalyzed by HRP, the working solution emits a specific wavelength of fluorescence (400-450nm), which can be exposed to X-ray films or directly scanned using fluorescence CCD. It is mainly used in Western detection and chemiluminescence immunoassay systems. The sensitivity of the hypersensitive type is more than 100 times higher than that of the enhanced type. For trace samples that cannot be detected by the enhanced type, the hypersensitive type can be selected.
Storage temperature:
Store at 2-8 ℃, do not freeze. Validity period: 2 years
Transportation conditions:
Ice bags.
Precautions:
1. For proteins with higher content (such as internal reference proteins), the luminescence intensity is high. The working solution can be diluted 2-5 times with deionized water to save color solution.
2. The reagent kit SolutionI serves as the substrate and is stored in a dark reagent bottle, while Solution II serves as the oxidant. The usual sampling sequence is to first take the substrate Solution I, change the gun head, and then take the oxidant Solution II. This can avoid forgetting to change the gun head, causing substrate failure.
3. This reagent kit is relatively stable and can be stored at room temperature (25 ℃) for more than a year. However, it is recommended to store it in a 4 ℃ refrigerator for long-term use. High temperatures in summer may cause reagent decomposition and reduce detection sensitivity.
4. When using the biotin avidin system, avoid using milk blocking, as it may cause a high background.
5. Metal oxide particles may cause granular spots on the film. Avoid using rusty scissors and tweezers. Instead, use flat headed plastic tweezers. If not, attach a gun head to the pointed tweezers.
6. Sodium azide inhibits the catalytic ability of HRP, and try to avoid using sodium azide as a preservative in the buffer solution.
7. Steps such as sealing, film washing, and incubation take a long time. Pay attention to the uneven friction between the film and plastic interface, which may cause some positions to disappear. You can incubate and seal in the cling film. The bottom surface of the plastic box for washing the film should not have obvious protrusions, and the protein side of the transfer film can be distinguished by cutting corners.
8. Different transfer membranes have different adsorption abilities for proteins, and nitrocellulose is softer to avoid creases; Before use, PVDF membranes need to be uniformly hydrated with methanol.
9. Do not place multiple films in the same film washing box for washing, as mutual adsorption and friction may leave traces.
10. Avoid bubbles during transfer, sealing, and incubation, which is the key to obtaining * * images.
11. Some fresh-keeping films have chemiluminescent quenching agents on them, please pay attention to their selection. Ordinary plastic wrap on the market may be too thin