LabScript RT Kit
This reagent kit is an efficient, stable, fast, and able to remove genomic DNA contamination reverse transcription system. The reagent kit adopts a new generation of reverse transcriptase LabScript M-MuLV RTase with multiple point mutations using molecular evolution technology, greatly improving stability and reverse transcription efficiency.
This kit is a one tube reverse transcription premixed reaction solution, 5 × RT MasterMix II contains all the reagents required for reverse transcription of the first strand (LabScript M-MuLV RTase, RNase Inhibitor, Random primers, Oligo dT Primer, dNTP Mixture, Buffer).
Usually, experiments such as Real Time RT-PCR require DNase I digestion to remove residual genomic DNA (gDNA) from RNA, but traditional DNase I treatment is complex and prone to RNA degradation and loss. Special 4 with DNA decomposition activity is used in this kit × GDNA Eraser Mix eliminates gDNA residues in 2 minutes without the need for DNase digestion and subsequent cumbersome steps.
First strand cDNA synthesis.
It can be used for the detection of high copy and low copy genes.
The new generation of reverse transcriptase LabScript M-MuLV RTase significantly improves stability and reverse transcription efficiency.
Using gDNA Eraser only takes 2 minutes to remove DNA residues, eliminating the need for DNase digestion and subsequent cumbersome steps.
Fully premixed reverse transcription Mix, simply add RNA and water to complete reverse transcription in 15 minutes.
At the same time, the two Mixes are not easy to freeze at -20 ℃, reducing the thawing and mixing time, making it easier to use.
This product specifically optimizes the oligo dT and N6 random primer ratio for qPCR, allowing cDNA synthesis to start from various regions of the RNA transcript and have the same reverse transcription efficiency, ensuring the authenticity and repeatability of qPCR results to the greatest extent possible.
Component 50 times 100 times
four × GDNA Eraser Mix 200 μ L 400 μ L
five × RT MasterMix II 200 μ L 400 μ L
five × No RTase Control Mix * 20 μ L 40 μ L
RNase free H2O 1mL 1mL
Note *: 5 × No RTase Control Mix contains no LabScript M-MuLV reverse transcriptase, other components, and 5 × RT MasterMix II is completely consistent and can be used as a negative control for reverse transcription.
Avoid RNase contamination.
To ensure successful reverse transcription, it is recommended to use high-quality RNA samples.
four × GDNA Eraser Mix, 5 × RT MasterMix II is very viscous, and the solution is easy to adsorb on the pipe wall and absorb