• LAMP SYBR Green Reagent(Lyophilized )
  • LAMP SYBR Green Reagent(Lyophilized )
  • LAMP SYBR Green Reagent(Lyophilized )
  • LAMP SYBR Green Reagent(Lyophilized )

LAMP SYBR Green Reagent(Lyophilized )

LAMP is a novel nucleic acid amplification technique that uses 4 or 6 primers that can recognize 6 specific regions on the target gene. It relies on the strong strand displacement activity of Bst DNA polymerase and can amplify DNA 109-1010 times within 30-60 minutes. This product requires real-time monitoring of LAMP amplification by adding SYBR Green dye. It requires a fluorescence quantitative PCR instrument or a constant temperature amplification fluorescence detector,
$4.50
  • LAMP SYBR Green Reagent(Lyophilized )
  • LAMP SYBR Green Reagent(Lyophilized )

SPECIFICATION

Product Introduction
LAMP is a novel nucleic acid amplification technique that uses 4 or 6 primers that can recognize 6 specific regions on the target gene. It relies on the strong strand displacement activity of Bst DNA polymerase and can amplify DNA 109-1010 times within 30-60 minutes.
This product requires real-time monitoring of LAMP amplification by adding SYBR Green dye. It requires a fluorescence quantitative PCR instrument or a constant temperature amplification fluorescence detector, and requires high primer requirements. High specificity primers can better avoid the occurrence of false positives.

Reagent components
NAME 100T×20μl
Lyophilized LAMP SYBR Green Reagent 1 bottle
2×PCR Buffer  1.5ml

Main features
The hot start enzyme adopts a proprietary modification method, with a 30 second hot start
Introducing UNG can efficiently eliminate aerosol pollution
Single component premixed solution, convenient and fast to use
Freeze dried products with extremely stable performance, suitable for long-term storage
One step qPCR quantitative reagent
Suitable for all real-time quantitative PCR instruments

application
Development of genetic testing and diagnostic reagents.

Storage
LAMP SYBR Green Reagent is in the form of dry powder. Before dissolution, the product can be stored for a long time at -20 ℃ and transported at room temperature without any degradation in performance. Before use, dissolve each bottle in 0.75ml of LAMP Buffer and use immediately. The remaining reagents can be stored at -20 ℃ for 1 month and stored for a long time below -60 ℃.
Reaction examples
example
Using AFSV standard plasmids and genomic DNA extracted from diseased materials as templates, this kit and 6 primers were used for LAMP SYBR Green reaction, with NTC as the control without a template. The results are shown in the figure.
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