Product use: Used for pathogenic Escherichia coli (including O157: H7/NM)
Identification test.
Principle of LST-MUG broth culture medium inspection: Tryptone provides carbon and nitrogen sources to meet the needs of bacterial growth; Sodium chloride can maintain a balanced osmotic pressure; Lactose is a fermentable sugar of the Escherichia coli group; Potassium dihydrogen phosphate and potassium dihydrogen phosphate are buffering agents; Sodium lauryl sulfate can inhibit the growth of non coliform bacteria. The glucuronidase contained in Escherichia coli acts on 4-methylumbelliferone- β- D-glucuronide- β- D-Glucuronide (MUG) β The glucuronic acid glycosidic bond is hydrolyzed, and the released 4-methylumbelliferone produces blue-white fluorescence under a 366nm ultraviolet lamp. 97% of Escherichia coli, 10% of Salmonella, and a small amount of Shigella have glucuronidase activity; Pathogenic Escherichia coli (including O157: H7) does not possess glucuronidase and therefore does not produce fluorescence.
Formula ingredients:
Formula (per liter) content
Tryptone 20.0g
Sodium chloride 5.0g
Lactose 5.0g
Dipotassium hydrogen phosphate 2.75g
Potassium dihydrogen phosphate 2.75g
Sodium lauryl sulfate 0.1g
MUG 0.1g
Final pH 6.8 ± 0.2
Usage: Take a bag of this product, add 50mL of distilled or deionized water, stir, heat, and boil until completely dissolved. Divide into test tubes with small inverted tubes, sterilize under high pressure at 121 ℃ for 15 minutes, let cool to room temperature, and set aside.
Quality control: The following quality control strains were inoculated into the culture medium to be tested at 44.5 ℃± 0.5 ℃, and the results were as follows after 24 hours: