• Lysine  Oxidase(LOX)
  • Lysine  Oxidase(LOX)

Lysine Oxidase(LOX)

1) Good water solubility; 2) High sensitivity; 3) Good linearity; 4) Good stability
Protein purity: ≥ 90%; Specific activity: ≥ 20 U/mg enzyme powder; Pyruvate oxidase: ≤ 0.001%; Choline oxidase: ≤ 0.001%; Uricase: ≤ 0.001%; Glucose oxidase: ≤ 0.001%
$10.00
  • Lysine  Oxidase(LOX)

SPECIFICATION

Technical indicators
Appearance: Yellow amorphous freeze-dried powder; Protein purity: ≥ 90%; Specific activity: ≥ 20 U/mg enzyme powder; Pyruvate oxidase: ≤ 0.001%; Choline oxidase: ≤ 0.001%; Uricase: ≤ 0.001%; Glucose oxidase: ≤ 0.001%
Enzymatic properties
Source: Microorganisms; Classification: EC 1.1.3.2; Molecular weight: 42kDa (SDS-PAGE); Isoelectric point: pH 4.6; Km value: 7.5 × 10-4 M (L-Lactate); Optimal pH: 6.0-7.0; Optimal temperature: 50 ° C; PH stability: pH 6.0-8.5 (25 ° C, 16 hours); Thermal stability: Stable below 50 ℃ (pH 7.0,30 min); Stability: Keep at -25~-15 ℃ for 12 months and maintain over 90% activity Purpose: Used for detecting lactate content

Definition of enzyme activity
Unit enzyme activity is defined as the catalytic production of 1 per minute under the following conditions μ The amount of enzyme required for mol H2O2.
Reagent preparation
Reagent I: 0.2 M pH 6.5 potassium phosphate buffer.
Reagent II: 1kU/mL peroxidase (POD) solution.
Reagent III: 50 mM 4-AA solution.
Reagent IV: 0.5 M DL lactic acid solution, pH 6.5.
Reagent V: 50 mM TOOS solution.
Enzyme diluent: 10 mM pH 7.0 Potassium phosphate buffer containing 10 μ M FAD.
Sample: Dilute the enzyme with enzyme diluent to 0.05-0.2U/mL.
Prepare the reaction mixture as follows: Reagent I: 10 ml; Reagent II: 0.25 mL; Reagent III: 1.5 mL; Reagent IV: 5 mL; Reagent V: 1.5 mL; Double distilled water replenished to 50 ml

Operating Steps
1. Add 1 mL of reaction mixture to a 1 mL cuvette.
2. Preheat the reaction mixture at 37 ° C for 5 minutes.
3. Add 20 μ L enzyme solution to be tested, mix well.
4. Measure the reaction at 37 ° C at 555 nm and record the absorbance change within 1 minute( Δ As).
*Replace the enzyme solution with enzyme diluent, and the other steps are the same. The absorbance of the obtained solution is blank absorbance( Δ Ab) Δ A= Δ As- Δ Ab
CONTACT US
FirstName*
LastName*
Email*
Message*