• Lyophilized ASFV LAMP Kit(Visual OTG Dye)
  • Lyophilized ASFV LAMP Kit(Visual OTG Dye)

Lyophilized ASFV LAMP Kit(TaqMan Probe)

Our company relies on a strong background in tool enzyme development to develop Bst DNA polymerase 5.0 with strong chain displacement ability, efficient DNA synthesis ability, and exonuclease activity. Whether using DNA as a template or RNA as a template, it can achieve amplification similar to TaqMan PCR method under constant temperature conditions of 60-65 ℃. The entire detection time is usually within 15-25 minutes to complete the detection. After applying this technology, based on the advant
$4.00
  • Lyophilized ASFV LAMP Kit(Visual OTG Dye)

SPECIFICATION

This kit is based on LAMP TaqMan technology and utilizes our unique Bst DNA polymerase technology. It has the advantages of LAMP fast, high sensitivity, and constant temperature detection, while overcoming the problem of LAMP prone to false positives.
LAMP is a new type of nucleic acid amplification technology, which has become a star potential technology for diagnostic reagents due to its rapid amplification ability. However, in nearly 20 years of development, it still cannot be widely applied in the field of diagnostic reagents. The prominent problem of LAMP technology is its severe false positive and non-specific amplification, which cannot generate highly specific signals. The application of TaqMan probe technology similar to standard quantitative PCR in LAMP detection has always been a goal pursued by molecular biologists.
Our company relies on a strong background in tool enzyme development to develop Bst DNA polymerase 5.0 with strong chain displacement ability, efficient DNA synthesis ability, and exonuclease activity. Whether using DNA as a template or RNA as a template, it can achieve amplification similar to TaqMan PCR method under constant temperature conditions of 60-65 ℃. The entire detection time is usually within 15-25 minutes to complete the detection. After applying this technology, based on the advantages of LAMP's fast, high sensitivity, and constant temperature detection, the problem of false positives in LAMP technology has been completely solved. The application of TaqMan probe in LAMP amplification system has laid a solid foundation for the wider application of LAMP technology in clinical diagnosis.

Kit features:
Quick detection: It can be completed within 15-25 minutes.
High sensitivity: Stable detection of single copies.
High specificity: Six specific primers and one TaqMan probe ensure the specificity of the product and the reliability of the detection.
Easy to operate: constant temperature detection.
Matching portable small devices, capable of field operation
Suitable for the detection of crude samples without the need for nucleic acid purification preparation.
Instrument and program settings:
The detection of LAMP TaqMan can be performed using a standard quantitative PCR instrument (or a constant temperature fluorescence device), with the probe reporting a FAM as the functional group.
The LAMP TaqMan amplification procedure using a quantitative PCR instrument is as follows:
Step 1: 60 ℃ for 10s
Step 2: Collect the signal at 60 ℃ for 50 seconds and cycle 25 times
The total reaction time is 25 minutes.

Usage:
Dissolve LAMP TaqMan freeze-dried products. Carefully open the aluminum cap of the penicillin bottle, add 1mL of LAMP TaqMan Buffer 2D to each bottle of freeze-dried products, and place it on a vortex mixer for 10 seconds to dissolve the freeze-dried products (or use a 1mL suction head to blow and mix evenly). The dissolved product can be immediately used or stored at -20 ℃. The dissolved reagent is labeled as LAMP TaqMan Mix.
Dissolve the positive standard, the positive standard is in dry powder form, and add 100% before first use μ L of ddH2O was dissolved in a vortex for 10 seconds and stored at -20 ℃. The concentration of the dissolved product is 105 copies/ μ l. Use 1 for each experiment μ L is sufficient.
Configuration reaction system
Dissolved LAMP TaqMan Mix 20 μ L
Detection template DNA 1 μ L
After the reaction system is prepared, it is thoroughly mixed and briefly centrifuged, and then placed on a fluorescence quantitative PCR instrument for reaction.

Result determination:
Conditions for the establishment of result interpretation
The negative water control does not peak, and the positive standard is effective in interpreting results under peak conditions of 10-14 minutes.
Positive result interpretation
If there is an amplification curve, it is determined to be positive. When the peak starts after 25 minutes but is not obvious, the number of copies is usually less than 2. A review experiment is required, and if the peak still appears, it indicates a positive result. Otherwise, it indicates negative.
Interpretation of negative results
The horizontal line indicates a negative sample.
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