• Lyophilized Fast DNA TaqMan PCR Kit(UNG)
  • Lyophilized Fast DNA TaqMan PCR Kit(UNG)
  • Lyophilized Fast DNA TaqMan PCR Kit(UNG)
  • Lyophilized Fast DNA TaqMan PCR Kit(UNG)

Lyophilized TaqMan RT-PCR Kit

Kit features: ·The hot start enzyme adopts a proprietary modification method, with a 30 second hot start; ·Dry powder products, performance and stability; ·Excellent amplification performance, strong tolerance to impurities in the sample; ·High sensitivity, capable of detecting low abundance genes; ·Suitable for all real-time quantitative PCR instruments.
$3.00
  • Lyophilized Fast DNA TaqMan PCR Kit(UNG)
  • Lyophilized Fast DNA TaqMan PCR Kit(UNG)

SPECIFICATION

This reagent kit is a specialized reagent for Real Time PCR using TaqMan probe method using RNA as a template. It is suitable for dual labeled probes such as FAM/HEX/TT/JOE/ROX (this product is also suitable for Molecular Beacon probes). The freeze-dried RT-PCR reagent uses hot start Fast Taq DNA polymerase, heat resistant reverse transcriptase, dNTPs, etc. to make freeze-dried product reagents. The product has stable performance and can be stored for a long time. When adding 2 × After the TaqMan Buffer is dissolved, the reagent becomes a conventional one-step probe method RT-PCR single component premixed reagent. When conducting experiments, the preparation of the PCR reaction solution is very convenient and simple, just adding primers, probes, RNA templates, and water. This product does not contain ROX correction dyes and is suitable for various fluorescent labeled probes. It is also suitable for various quantitative PCR models and is commonly used for relative quantitative detection of RNA.
The heat-resistant reverse transcriptase in this freeze-dried RT-PCR reagent exhibits excellent thermal stability, retaining over 90% polymerization activity at 55 ℃. The Fast Taq DNA polymerase in the product uses proprietary hot start technology to ensure that there is no activity below 55 ℃, so a reaction system can be established at room temperature. Fast Taq DNA polymerase has excellent amplification speed, therefore it meets the requirements of fast PCR programs and can usually complete the amplification reaction within 30 minutes. At the same time, this polymerase has the ability to tolerate impurities and is also suitable for amplification reactions of crude nucleic acid samples

Kit features:
·The hot start enzyme adopts a proprietary modification method, with a 30 second hot start;
·Dry powder products, performance and stability;
·Excellent amplification performance, strong tolerance to impurities in the sample;
·High sensitivity, capable of detecting low abundance genes;
·Suitable for all real-time quantitative PCR instruments.

Usage:
1. Dissolved freeze-dried products
Add 1ml of TaqMan PCR Buffer 7 to each bottle to dissolve the lyophilized product. The dissolved product is a TaqMan PCR Mix reagent (2 times the concentration) × TaqMan PCR Mix). The remaining reagents after dissolution can be stored at -20 ℃, and can be used directly after melting in the next experiment.
2. Configure reaction system
Prepare 20 according to the following components μ PCR reaction system

Prepare 20 according to the following components μ PCR reaction system  

Ingredients

Dosage

concentration

2×TaqMan PCR Mix(溶解后)

10μl

PCR Forward Primer (10μM)

0.4μl

0.2μM

PCR Reverse Primer (10μM)

0.4μl

0.2μM

TaqMan Probe (10μM)

0.4μl

0.2μM

cDNADNA

0.5~2μl

ddH2O

up to 20μl


3.   Perform Real Time PCR reaction using the following procedure: 



Temperature

Time

Cycles

Explain

55℃

10min

1

RT

95℃

35s

1

Hot start reaction

95℃

5s

40

60

20s

 

 

 

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