• African Swine Fever Real-time Fluorescence PCR Detection Kit(Lyophilized)
  • African Swine Fever Real-time Fluorescence PCR Detection Kit(Lyophilized)
  • African Swine Fever Real-time Fluorescence PCR Detection Kit(Lyophilized)
  • African Swine Fever Real-time Fluorescence PCR Detection Kit(Lyophilized)

African Swine Fever Real-time Fluorescence PCR Detection Kit(Lyophilized)

1. The product is in the form of freeze-dried products, stored and transported at any temperature, with a long shelf life 2. The reagents, primers, and probes are all in one tube, which is extremely simple and convenient to use 3. The hot start Fast Taq DNA polymerase paired with the reagent kit ensures rapid amplification speed, completing the detection within 30 minutes 4. The detection sensitivity reaches a stable level of 5 Copy, and the extracted nucleic acid samples of diseased material
$3.50
  • African Swine Fever Real-time Fluorescence PCR Detection Kit(Lyophilized)
  • African Swine Fever Real-time Fluorescence PCR Detection Kit(Lyophilized)

SPECIFICATION

Product Introduction
Lyophilized Fast DNA TaqMan PCR Reagent is a specialized reagent for Real Time PCR using the TaqMan probe method. It is suitable for dual labeled probes such as FAM/HEX/TT/JOE/ROX (this product is also suitable for Molecular Beacon probes). The freeze-dried TaqMan PCR Reagent uses hot start Fast Taq DNA polymerase, dNTP, etc. to make freeze-dried product reagents, which have stable performance and can be stored for a long time.
When adding 2 × After the TaqMan Buffer is dissolved, the reagent becomes a conventional TaqMan PCR Mix single component premixed reagent. When conducting experiments, the preparation of the PCR reaction solution is very convenient and simple, simply adding primers, probes, DNA or cDNA templates, and water. This product does not contain ROX correction dyes and is suitable for various fluorescent labeling probes, as well as for various quantitative PCR models.
The Fast Taq DNA polymerase in this product uses proprietary hot start technology to ensure that there is no activity below 55 ℃, so a reaction system can be established at room temperature. Fast Taq DNA polymerase has excellent amplification speed, therefore it meets the requirements of fast PCR programs and can usually complete the amplification reaction within 30 minutes. At the same time, this polymerase has the ability to tolerate impurities and is also suitable for amplification reactions of crude nucleic acid samples.

Reagent components
NAME 100T×20μl
Lyophilized DNA TaqMan PCR Reagent 1 bottle
2×TaqMan PCR Buffer5 1ml 

Main advantages
1. The product is in the form of freeze-dried products, stored and transported at any temperature, with a long shelf life
2. The reagents, primers, and probes are all in one tube, which is extremely simple and convenient to use
3. The hot start Fast Taq DNA polymerase paired with the reagent kit ensures rapid amplification speed, completing the detection within 30 minutes
4. The detection sensitivity reaches a stable level of 5 Copy, and the extracted nucleic acid samples of diseased materials can be as low as the "Fick" level, providing a reliable solution for the detection of samples such as swabs, water, and environment
5. While ensuring high sensitivity, extremely strict negative control levels are in place to avoid false positive detection and facilitate the interpretation of results by testing personnel
6. The primer probe covers the conserved outer membrane protein P72 region of African swine fever to avoid missed detection of mutated viruses
7. Advanced LNA short primer technology provides the most rigorous and rigorous primer probe combination, ensuring the high reliability of the reagent kit

Storage
1. Lyophilized ASFV TaqMan PCR Reagent is in the form of freeze-dried products, containing hot start Fast Taq DNA polymerase, dNTP, amplification primers, and probes. Each bottle can be used for 50 amplification reactions.
2. Lyophilized ASFV TaqMan PCR Reagent can be stored at room temperature for 6 months, 4 ℃ for 12 months, -20 ℃ for long-term storage, and can be transported at room temperature.
3. Freeze dried products undergo 1 × After TaqMan PCR Buffer2 is dissolved, it can be stored for a long time at -20 ℃.
4.1 × TaqMan PCR Buffer2 can be stored at room temperature for a long time, but is usually stored at 4 ℃ or -20 ℃.

Reaction examples
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