• Bst 4.0 LAMP Lyophilized Bead and Mix
  • Lyophilized Fast DNA TaqMan PCR Reagent (UNG)
  • Bst 4.0 LAMP Lyophilized Bead and Mix
  • Lyophilized Fast DNA TaqMan PCR Reagent (UNG)

Lyophilized Fast DNA TaqMan PCR Reagent (UNG)

1.Lyophilized Fast DNA TaqMan PCR Reagent is a specialized reagent for Real Time PCR using the TaqMan probe method. It is suitable for dual labeled probes such as FAM/HEX/TT/JOE/ROX. The Lyophilized TaqMan PCR Reagent uses hot start Fast Taq DNA polymerase, dNTP, etc. to make freeze-dried product reagents, which have stable performance and can be stored for a long time. 2.The UNG enzyme in this reagent can eliminate up to 100000 copies of nucleic acid pollutants by incubating at 37 ° C for 2 mi
$3.00
  • Bst 4.0 LAMP Lyophilized Bead and Mix
  • Lyophilized Fast DNA TaqMan PCR Reagent (UNG)

SPECIFICATION

Product Introduction
Due to the extremely high amplification efficiency of PCR for nucleic acids, it is very easy to cause aerosol pollution in diagnostic PCR experiments, such as pipettes, countertops, PCR instruments, and air. When the experimental environment is contaminated, it can lead to false positive results for negative samples. In this reagent, all dTTPs in dNTP are replaced with dUTP, so that the amplified PCR product contains dU bases. In subsequent experiments, dU PCR products containing aerosol contamination will be degraded by UNG glycosylase in this reagent, thereby removing the aerosol contamination effect. The UNG enzyme in this reagent can eliminate up to 100000 copies of nucleic acid pollutants by incubating at 37 ° C for 2 minutes. Therefore, this product is the best reagent for diagnostic quantitative PCR detection.
Lyophilized Fast DNA TaqMan PCR Reagent is a specialized reagent for Real Time PCR using the TaqMan probe method. It is suitable for dual labeled probes such as FAM/HEX/TT/JOE/ROX (this product is also suitable for Molecular Beacon probes). The freeze-dried TaqMan PCR Reagent uses hot start Fast Taq DNA polymerase, dNTP, etc. to make freeze-dried product reagents, which have stable performance and can be stored for a long time.
When adding 2 × After the TaqMan Buffer is dissolved, the reagent becomes a conventional TaqMan PCR Mix single component premixed reagent. When conducting experiments, the preparation of the PCR reaction solution is very convenient and simple, simply adding primers, probes, DNA or cDNA templates, and water. This product does not contain ROX correction dyes and is suitable for various fluorescent labeling probes, as well as for various quantitative PCR models.
The Fast Taq DNA polymerase in this product uses proprietary hot start technology to ensure that there is no activity below 55 ℃, so a reaction system can be established at room temperature. Fast Taq DNA polymerase has excellent amplification speed, therefore it meets the requirements of fast PCR programs and can usually complete the amplification reaction within 30 minutes. At the same time, this polymerase has the ability to tolerate impurities and is also suitable for amplification reactions of crude nucleic acid samples.

Reagent components
NAME 200T×20μl
Lyophilized Fast DNA TaqMan PCR Reagent 2 bottle
2×TaqMan PCR Buffer5 1ml×2

Main features
The hot start enzyme adopts a proprietary modification method, with a 30 second hot start
Dry powder products, performance and stability
Excellent amplification performance and strong tolerance to impurities in the sample
High sensitivity, able to detect low abundance genes
Suitable for all real-time quantitative PCR instruments
application
Relative quantitative detection of DNA and cDNA.
matters needing attention
Storage
1. Lyophilized Fast DNA TaqMan PCR Reagent is in dry powder form and can be used for 100 amplification reactions per bottle.
2. Lyophilized Fast DNA TaqMan PCR Reagent is in dry powder form and can be stored at room temperature for 12 months, long-term storage at -20 ℃, and can be transported at room temperature.
3. Freeze dried products undergo 2 × After TaqMan PCR Buffer5 is dissolved, it can be stored for a long time at -20 ℃.

Reaction examples
Fig. Using the pUC57 plasmid as a template, the template was subjected to 8 4-fold dilutions, and the copy number of the template in the 8th dilution gradient was 10 copies/ μ l. Amplification detection using Fast DNA TaqMan PCR Reagent (UNG), with template 2 added to each reaction system μ l. As shown in the figure,
A: This product exhibits excellent linear relationship within a wide range of template quantities: amplification efficiency of 97.5%, R2=0.9995.
B: To test the ability of UNG to remove DNA contamination, 105 copies of dUTP containing PCR products were added to the reaction system. Quantitative PCR was performed using ordinary freeze-dried Fast DNA TaqMan PCR Reagent (B1) and Fast DNA TaqMan PCR Reagent (UNG) (B2), respectively. From the figure, it can be seen that UNG can effectively remove 105 copies of aerosol pollution.
C: The standard curve of Figure A amplification.
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