Main features
The hot start enzyme adopts a proprietary modification method, with a 30 second hot start
Introducing UNG can efficiently eliminate aerosol pollution
Single component premixed solution, convenient and fast to use
Freeze dried products with extremely stable performance, suitable for long-term storage
One step TaqMan qPCR quantitative reagent
Suitable for all real-time quantitative PCR instruments
application
Development of genetic testing and diagnostic reagents.
Storage
1. Lyophilized Fast RNA TaqMan PCR Reagent (UNG) is in dry powder form and can be used for 100 amplification reactions per bottle.
2. Lyophilized Fast RNA TaqMan PCR Reagent (UNG) is a dry powder form that can be stored at room temperature for 12 months, long-term storage at -20 ℃, and can be transported at room temperature.
3. Freeze dried products undergo 2 × After dissolution of TaqMan PCR Buffer, it can be stored for a long time at -20 ℃.
Fig. Using the pUC57 plasmid as a template, the template was subjected to 8 4-fold dilutions, and the copy number of the template in the 8th dilution gradient was 10 copies/ μ l. Amplification detection using Fast DNA TaqMan PCR Reagent (UNG), with template 2 added to each reaction system μ l. As shown in the figure,
A: This product exhibits excellent linear relationship within a wide range of template quantities: amplification efficiency of 97.5%, R2=0.9995.
B: To test the ability of UNG to remove DNA contamination, 105 copies of dUTP containing PCR products were added to the reaction system. Quantitative PCR was performed using ordinary freeze-dried Fast DNA TaqMan PCR Reagent (B1) and Fast DNA TaqMan PCR Reagent (UNG) (B2), respectively. From the figure, it can be seen that UNG can effectively remove 105 copies of aerosol pollution.
C: The standard curve of Figure A amplification.
