This reagent kit is based on LAMP-OTG color change amplification technology. Compared to liquid products on the market, freeze-dried products are more stable, sensitive, and easy to operate. The detection is usually completed within 30 minutes. The kit contains Bst4.0 DNA polymerase, which can amplify both DNA and RNA molecules directly.
Orange to Green color changing dye OTG (Orange to Green) is pre added to the 8-tube cap in a freeze-dried form. After the LAMP reaction is completed (before the reaction is completed, the dye on the tube cover must not be mixed with the reaction solution), invert the 0.2mL EP tube to dissolve the OTG dye. The reaction product of LAMP will form a strong green visible discoloration reaction with OTG dye (positive), while the EP tube that has not undergone amplification will be dark orange (negative).
Kit features:
·The hot start enzyme adopts a proprietary modification method, with a 30 second hot start;
·Dry powder products, performance and stability;
·Excellent amplification performance, strong tolerance to impurities in the sample;
·High sensitivity, capable of detecting low abundance genes;
·Suitable for all real-time quantitative PCR instruments.
Usage:
1. Dissolved freeze-dried products
Lyophilized LAMP OTG Reagent is a freeze-dried powder containing Bst DNA polymerase 4.0, dNTPs, etc. Before dissolution, the product can be stored for a long time at -20 ℃ and transported at room temperature without any degradation in performance. After dissolving each bottle with 0.75mL LAMP Buffer before use, it can be used immediately. The remaining reagents can be stored at -20 ℃ for 1 month and stored for a long time below -60 ℃.
2. Configure reaction system
Prepare 20 according to the following components μ PCR reaction system
2.The setting of the fluorescence quantitative PCR instrument LAMP OTG can be detected using a standard quantitative PCR instrument (or a constant temperature fluorescence device).
Using a quantitative PCR instrument for LAMP OTG amplification program:
Step 1: 60 ℃ for 10s
Step 2: Collect the signal at 60 ℃ for 50 seconds and cycle 25 times
The total reaction time is 25 minutes.
Note: It is prohibited to open the reaction tube cover after the LAMP reaction is completed, otherwise it may cause aerosol pollution. Once aerosol pollution occurs, please use our DNA aerosol pollution remover (product number: MT3001) for environmental cleaning.
