• Human Liver Ductal Organoid Kit (Differentiation)
  • Human Liver Ductal Organoid Kit (Differentiation)
  • Mouse Liver Ductal Organoid Kit (Differentiation)
  • Mouse Liver Ductal Organoid Kit (Differentiation)
  • Human Liver Ductal Organoid Kit (Differentiation)
  • Human Liver Ductal Organoid Kit (Differentiation)
  • Mouse Liver Ductal Organoid Kit (Differentiation)
  • Mouse Liver Ductal Organoid Kit (Differentiation)

Mouse Liver Ductal Organoid Kit (Differentiation)

Mouse Liver Ductal Organoid Kit is a chemically defined cell culture medium for establishment and maintenance of Mouse ductal organoids(mLDs) derived from adult stem cells. Self-renewal of the ductal epithelium is driven by the proliferation of stem cells and their progenitors located in liver. mLDs display all hallmarks of the ductal epithelium in terms of architecture, cell type composition, and self-renewal dynamics, therefore hold great promise for unprecedented studies of Mouse liver development and disease, mLDs could differentiate into hepatocyte like cell under the induction of differentiation medium.
$820.00
  • Human Liver Ductal Organoid Kit (Differentiation)
  • Human Liver Ductal Organoid Kit (Differentiation)
  • Mouse Liver Ductal Organoid Kit (Differentiation)
  • Mouse Liver Ductal Organoid Kit (Differentiation)

SPECIFICATION

Organoids refer to tissue analogues with a certain spatial structure formed by three-dimensional cultivation of adult stem cells or pluripotent stem cells in vitro. Organs are highly consistent with their source tissues in terms of tissue structure, cell type, self-renewal ability, and function, thus demonstrating unique advantages in multiple biomedical fields such as developmental biology, disease modeling, precision medicine, drug research and development, gene and cell therapy, infection and immunity, and regenerative medicine.

Product Description:

Mouse Liver Ductal Organoid Kit is a chemically defined cell culture medium for establishment and maintenance of Mouse ductal organoids(mLDs) derived from adult stem cells. Self-renewal of the ductal epithelium is driven by the proliferation of stem cells and their progenitors located in liver. mLDs display all hallmarks of the ductal epithelium in terms of architecture, cell type composition, and self-renewal dynamics, therefore hold great promise for unprecedented studies of Mouse liver development and disease, mLDs could differentiate into hepatocyte like cell under the induction of differentiation medium.

Product Information:

Component

Component Cat#

Volume

Storage& Stability

 Mouse Liver Ductal Organoid Basal Medium(Differentiation)

K2006-MLH –A100/A500

100mL/500mL

4℃,12 months

 Mouse Liver Ductal Organoid Supplement B(50x)(Differentiation)

K2006-MLH –B100/B500

2mL/10mL

-20℃,avoid repeated freeze-thaw cycles, 12 months

 Mouse Liver Ductal Organoid Supplement C(250x) (Differentiation)

K2006-MLH –C100/C500

0.4mL/2mL

-20℃, avoid repeated freeze-thaw cycles, 12 months

 Mouse Liver Ductal Organoid Supplement D(250x) (Differentiation)

K2006-MLH –D100/D500

0.4mL/2mL

-20℃, avoid repeated freeze-thaw cycles, 12 months

 

Preparation of Mouse Liver Ductal Organoid Differentiation Medium
Use sterile technique to prepare the mouse liver ductal organoid differentiation medium. mLDs grown in Mouse Liver Ductal Organoid Expansion Medium overwhelmingly consisted of cholangiocytes. After changing the Expansion Medium to differentiation medium, the mLDs could differentiate into hepatocyte like cells, which display the markers of hepatocytes, including Albumin, Ttr, Cyp3a11 and Mup20. The following examples are for preparing 10 mL of Differentiation I Medium and Differentiation II Medium. If preparing other volumes, adjust accordingly.
1.       Thaw Mouse Liver Ductal Organoid Supplement B(50x) (Differentiation), Mouse Liver Ductal Organoid Supplement C(250x) (Differentiation) and Mouse Liver Ductal Organoid Supplement D(250x) (Differentiation) on ice.
NOTE: Once thawed, use immediately or aliquot and store at -20°C for no more than 10 months. After thawing the aliquots, use immediately. Do not re-freeze.
2.       For Mouse Liver Ductal Organoid Differentiation Medium I. Add 200 μL Mouse Liver Ductal Organoid Supplement B(50x) (Differentiation), 40 μL Mouse Liver Ductal Organoid Supplement C(250x) (Differentiation) to 10 mL Mouse Liver Ductal Organoid Basal Medium (Differentiation). Mix thoroughly.
3.       For Mouse Liver Ductal Organoid Differentiation Medium II. Add 200 μL Mouse Liver Ductal Organoid Supplement B(50x) (Differentiation)and 40 μL Mouse Liver Ductal Organoid Supplement C(250x) (Differentiation) and 10 μL Mouse Liver Ductal Organoid Supplement D (250x) (Differentiation) to 10 mL Mouse Liver Ductal Organoid Basal Medium (Differentiation). Mix thoroughly.
NOTE: If not use immediately, store complete medium at 2-8°C for no more than 2 weeks. bioGenousTM Mouse Liver Ductal Organoid Supplement B (Differentiation) contains fungicide and antibiotics(50x). 
Protocol for Mouse Liver Ductal Organoids Differentiation
1.     Culture the mouse ductal organoids in Mouse Liver Ductal Organoid Medium(Expansion) (K2006-MLD) for at least 3 days.
2.     Change the medium to Mouse Liver Ductal Organoid Differentiation Medium I, and culture for 9 days. During this period, replace the medium every day.
3.     Change the medium to Mouse Liver Ductal Organoid Differentiation Medium II, and culture for 3 days. During this period, replace the medium every day.
4.     At the end of this period, the differentiation process is completed. The ductal organoid would differentiate into hepatocyte-like organoid with the expression of hepatic markers, such as Albumin, Ttr, Cyp3a11 and Mup20.

CONTACT US
FirstName*
LastName*
Email*
Message*