Product Background:
Glycation is one of the most common post-translational modifications of proteins in eukaryotic cells. According to incomplete estimates, over 50% of human proteins undergo glycosylation modifications, and 100% of the most eye-catching monoclonal antibodies in biopharmaceuticals are glycoprotein drugs. Therefore, research on protein glycosylation is becoming increasingly important.
N-glycosidic linked glycoproteins are the main glycosylation type in glycoproteins. Numerous studies have shown that the N-glycosylation modification of monoclonal antibodies has certain effects on their stability, clearance rate, immunogenicity, antibody dependent cytotoxicity (ADCC) and complement dependent cytotoxicity (CDC).
The problems faced by N-sugar analysis
The difficulty of sample processing is high: Antibody glycotype analysis requires complex pre-treatment steps on the sample, which require a lot of time and resources, and are easily affected by the source and quality of the sample.
Complex sugar type structure: The structure of antibody sugar types is relatively complex, including different types and positions of sugar modification, which makes sugar type analysis more difficult and requires highly professional technology and experience.
Insufficient standardization and consistency: Due to the fact that antibody glycotype analysis involves multiple steps and operations between multiple laboratories, there is a lack of standardized analysis methods and techniques, which leads to insufficient consistency in results and limits the development and application of antibody glycotype analysis.
Difficulty in data processing and interpretation: Antibody glycotype analysis generates a large amount of data, and data processing and interpretation are also complex, requiring professional software and algorithm support, which is a challenge for some small and medium-sized enterprises or research laboratories.
Product features:
For rapid enzymatic release and labeling of N-sugars
Paired with four modules, the operation process is simple
Operation time only takes 30 minutes
Accurate molecular weight labeling
Sample usage as low as 15 μ G
Provide 24&96T different testing specifications
Provide two purification methods: solid phase extraction column and 96 well extraction plate