NA-MUG Medium
NA-MUG Medium is a selective differential medium adopted for the detection of Escherichia coli in drinking water and source water by membrane filtration method.
Product Specifications
Packaging: 100 g/bottle
Shelf Life: 3 years
Composition (g/L)
Peptone 5.0
Beef extract powder 3.0
Agar 15.0
MUG 0.1
pH: 6.8 ± 0.2 at 25 ℃
Test Principle
Peptone and beef extract powder supply nitrogen sources, vitamins, amino acids and carbon sources. Agar works as the gelling agent. Glucuronidase from E. coli hydrolyzes the β-glucuronide bond of 4-methylumbelliferyl-β-D-glucuronide (MUG). The released 4-methylumbelliferone shows blue-white fluorescence under 366 nm ultraviolet lamp. Around 97% of E. coli, 10% of Salmonella, and a small portion of Shigella strains have glucuronidase activity.
Directions for Use
Weigh 23.1 g of medium powder, heat and stir to dissolve in 1000 mL distilled water. Autoclave at 121 ℃ for 15 minutes and reserve for use. Poured plates can be stored at 4 ℃ for up to two weeks.
Performance Test for NA-MUG Medium (100 g)
Prepare the medium following labelled instructions. Inoculate specified quality control strains and incubate aerobically at 36 ± 1 ℃ for 24–48 hours.
Note
TSA agar is used as the control medium when calculating recovery rate.
