Product Information:
|
Component |
Component Cat# |
Volume |
Storage& Stability |
|
Nasopharyngeal Carcinoma Organoid Kit Nasopharyngeal Carcinoma Organoid Kit |
K2006-MLH –A100/A500 |
100mL/500mL |
4℃，12 months |
|
Nasopharyngeal Carcinoma Organoid Supplement B(50x)（Differentiation） |
K2006-MLH –B100/B500 |
2mL/10mL |
-20℃,avoid repeated freeze-thaw cycles, 12 months |
|
Nasopharyngeal Carcinoma Organoid Supplement C(250x) （Differentiation） |
K2006-MLH –C100/C500 |
0.4mL/2mL |
-20℃, avoid repeated freeze-thaw cycles, 12 months |
|
Nasopharyngeal Carcinoma Organoid Supplement D(250x) （Differentiation） |
K2006-MLH –D100/D500 |
0.4mL/2mL |
-20℃, avoid repeated freeze-thaw cycles, 12 months |
Preparation ofNasopharyngeal Carcinoma Organoid Differentiation Medium
Use sterile technique to prepare theNasopharyngeal Carcinoma organoid differentiation medium. mLDs grown inNasopharyngeal Carcinoma Organoid Expansion Medium overwhelmingly consisted of cholangiocytes. After changing the Expansion Medium to differentiation medium, the mLDs could differentiate into hepatocyte like cells, which display the markers of hepatocytes, including Albumin, Ttr, Cyp3a11 and Mup20. The following examples are for preparing 10 mL of Differentiation I Medium and Differentiation II Medium. If preparing other volumes, adjust accordingly.
1. ThawNasopharyngeal Carcinoma Organoid Supplement B(50x) (Differentiation),Nasopharyngeal Carcinoma Organoid Supplement C(250x) (Differentiation) andNasopharyngeal Carcinoma Organoid Supplement D(250x) (Differentiation) on ice.
NOTE: Once thawed, use immediately or aliquot and store at -20°C for no more than 10 months. After thawing the aliquots, use immediately. Do not re-freeze.
2. ForNasopharyngeal Carcinoma Organoid Differentiation Medium I. Add 200 μLNasopharyngeal Carcinoma Organoid Supplement B(50x) (Differentiation), 40 μLNasopharyngeal Carcinoma Organoid Supplement C(250x) (Differentiation) to 10 mLNasopharyngeal Carcinoma Organoid Basal Medium (Differentiation). Mix thoroughly.
3. ForNasopharyngeal Carcinoma Organoid Differentiation Medium II. Add 200 μLNasopharyngeal Carcinoma Organoid Supplement B(50x) （Differentiation）and 40 μLNasopharyngeal Carcinoma Organoid Supplement C(250x) (Differentiation) and 10 μLNasopharyngeal Carcinoma Organoid Supplement D (250x) (Differentiation) to 10 mLNasopharyngeal Carcinoma Organoid Basal Medium (Differentiation). Mix thoroughly.
NOTE: If not use immediately, store complete medium at 2-8°C for no more than 2 weeks. bioGenousTMNasopharyngeal Carcinoma Organoid Supplement B (Differentiation) contains fungicide and antibiotics(50x).
Protocol forNasopharyngeal Carcinoma Organoids Differentiation
1. Culture the mouse ductal organoids inNasopharyngeal Carcinoma Organoid Medium(Expansion) (K2006-MLD) for at least 3 days.
2. Change the medium toNasopharyngeal Carcinoma Organoid Differentiation Medium I, and culture for 9 days. During this period, replace the medium every day.
3. Change the medium toNasopharyngeal Carcinoma Organoid Differentiation Medium II, and culture for 3 days. During this period, replace the medium every day.
4. At the end of this period, the differentiation process is completed. The ductal organoid would differentiate into hepatocyte-like organoid with the expression of hepatic markers, such as Albumin, Ttr, Cyp3a11 and Mup20.
