One-tube RT Kit

Name: One-tube RT Kit
Brand: BEYONDBIO-TECH: Your Integrated Platform & Supplier for Lab Instrument
Price: 1.70 USD
Availability: InStock

One-tube RT Kit is a ready-to-use one-tube reverse transcription premix for first-strand cDNA synthesis. It contains MMLV reverse transcriptase, random primers, Oligo dT primer, dNTP mix and reaction buffer. Only template RNA and RNase-free water are needed. It finishes cDNA synthesis in 20 min at 42 ℃, supports templates with high GC and complex secondary structures, up to 12 kb long cDNA. The obtained cDNA is compatible with qPCR. Store at -20 ℃, shelf life 1 year.

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SPECIFICATION

One-tube RT Kit

This one-tube reverse transcription premix contains all reagents needed for first-strand cDNA synthesis, including MMLV reverse transcriptase, random primers, Oligo dT primer, dNTP Mixture, reaction buffer and so on. Users only add template RNA and RNase-free water to set up the reaction.

Product Features

Convenient cDNA synthesis: only template RNA and RNase-free water are required.
Simplified workflow, reduces contamination and experimental errors.
High reverse transcription efficiency with optimized ratio of Oligo dT and N6 random primers.
Fast protocol: complete first-strand cDNA synthesis at 42 ℃ within 20 minutes.
Works well for RNA templates with high GC content and complex secondary structures; generates cDNA up to 12 kb.
Excellent compatibility: the synthesized cDNA can be directly applied in fluorescent quantitative PCR with high sensitivity and good stability.

Kit Contents

表格

Component	Specification
2× RT MasterMix	0.1 mL
RNase Free Water	1 mL
Instruction Manual	1 copy

Storage

Transport under low temperature; store at -20 ℃. Shelf life: 1 year.

Directions for Use

Thaw all components, invert and mix thoroughly. Take the 20 μL system as an example.

Note: 2× RT MasterMix is highly viscous. Briefly centrifuge before use to avoid loss on pipette tip walls.

Component	Volume
2× RT MasterMix	10 μL
Total RNA / mRNA template	50 ng–2 μg / 5–200 ng
RNase Free water	Adjust to 20 μL

For RNA templates with complex secondary structures, denaturation is recommended: incubate template RNA at 65 ℃ for 5 min, then immediately chill on ice before setup.

Mix gently and incubate at 42 ℃ for 20 min for reverse transcription.

Note: For templates with complex secondary structures or high GC content, raise the temperature to 50 ℃ to improve efficiency.

Inactivate reverse transcriptase at 85 ℃ for 5 sec, then keep on ice for subsequent use. Store at -20 ℃ for long-term preservation.

Precautions

Prevent RNase contamination to avoid RNA degradation and cross-contamination.
Thaw and mix completely before use; uneven salt concentration will affect results.
Return to -20 ℃ promptly after use; avoid repeated freeze-thaw cycles.
RNA integrity is critical for cDNA synthesis efficiency; use reliable RNA extraction and purification methods.
For long fragments or structurally complex RNA, pre-incubate RNA separately at 65–70 ℃ for 5–10 min before adding into the reaction system to boost performance.
Wear masks and disposable gloves during operation.