Organoids refer to tissue analogues with a certain spatial structure formed by three-dimensional cultivation of adult stem cells or pluripotent stem cells in vitro. Organs are highly consistent with their source tissues in terms of tissue structure, cell type, self-renewal ability, and function, thus demonstrating unique advantages in multiple biomedical fields such as developmental biology, disease modeling, precision medicine, drug research and development, gene and cell therapy, infection and immunity, and regenerative medicine.
DESCRIPTIONBackground:Epidermal growth factor (EGF) is a small, potent growth factor capable of inducing cell proliferation, differentiation, and survival. EGF is the founding member of the EGF family which are characterized by a shared structural motif, the EGF- like domain. EGF is a high affinity ligand of the EGF receptor (ErbB). Four ErbB (HER) family receptor tyrosine kinases including EGFR/ErbB1, ErbB2, ErbB3 and ErbB4, mediate responses to EGF family members EGF binding induces dimerization of the EGF receptor resulting in activation of the protein tyrosine kinase signaling pathway. These receptors undergo a complex pattern of ligand-induced homo- or hetero-dimerization to transduce EGF family signals. Dimerization results in autophosphorylation of the receptor at specific tyrosine residues to create docking sites for a variety of signaling molecules. Biological activities ascribed to EGF include epithelial development, angiogenesis, fibroblast proliferation, and colony formation of epidermal cells in culture.
Product parameters
Source:E.coli
Protein Construction:A DNA sequence encoding the amino acids (Asn971-Arg1021) of human EGF (Accession Number: P01133) was expressed.
Synonyms:hEGF; HOMG4; beta-urogastrone; epidermal growth factor; pro-epidermal growth factor; URG; Urogastrone
SPECIFICATIONS
Purity:≥ 98%, by SDS-PAGE visualized with quantitative densitometry by Coomassie® Blue Staining.
Biological Activity:Measured in a cell proliferation assay using Balb/C 3T3 mouse embryonic fibroblast cells. The ED50 for this effect is typically 0.3-1.5 ng/mL.
Endotoxin Level:<0.01 EU per 1 μg of the protein by the LAL method
Calculated Molecular Weight:5.95 kDa
SDS-PAGE:8 kDa, reducing conditions
FORMULATION AND STORAGE
Formulation:The product is Lyophilized from a 0.22 μm filtered solution in PBS.
Shipping:The product is shipped on ice. Upon receipt, store it immediately as methods recommended below.
Reconstitution:Reconstitute in sterile PBS buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.
Stability & Storage:
24 months, -20 to -70 °C, under powder state;
12 months, -20 to -70 °C, under sterile conditions after reconstitution;
2 month, 2 to 8 °C under sterile conditions after reconstitution;
avoid repeated freeze-thaw cycles.