Organoids refer to tissue analogues with a certain spatial structure formed by three-dimensional cultivation of adult stem cells or pluripotent stem cells in vitro. Organs are highly consistent with their source tissues in terms of tissue structure, cell type, self-renewal ability, and function, thus demonstrating unique advantages in multiple biomedical fields such as developmental biology, disease modeling, precision medicine, drug research and development, gene and cell therapy, infection and immunity, and regenerative medicine.
Product Description:
Background:FGF-10 belongs to the subgroup of FGFs that also includes FGF-3, -7, and -22. FGF-10 is secreted by mesenchymal cells and associates with extracellular FGF-BP. It preferentially binds and activates epithelial cell FGF R2 (IIIb) and interacts more weakly with FGF R1 (IIIb) . The expression and function of FGF-10 are negatively regulated by Shh and BMP-4 in the developing lung . Overlapping expression patterns and activities with FGF-3, -7, and -8 suggest at least a partial redundancy in FGF‑10 biology. FGF-10 induced signaling through FGF R2 (IIIb) also contributes to the progression of pancreatic cancer .
Product parameters
Source:E.coli
Protein Construction:A DNA sequence encoding the amino acids (Gln38-Ser208) of human FGF10 (Accession Number: O15520) was expressed.
Synonyms:Fibroblast growth factor 10; Keratinocyte growth factor 2; KGF2; KGF-2. Purity:≥ 95%, by SDS-PAGE visualized with quantitative densitometry by Coomassie® Blue Staining.
Biological Activity:Measured in a cell proliferation assay using human liver ductal organoids. The ED50 for this effect is 20-100 ng/ml.
Endotoxin Level:<0.10 EU per 1 μg of the protein by the LAL method
Calculated Molecular Weight:19.5 kDa
SDS-PAGE:25 kDa, reducing condition
Formulation:The product is Lyophilized from a 0.22μm filtered solution in PBS.
Shipping:The product is shipped on ice. Upon receipt, store it immediately as methods recommended below.
Reconstitution:Reconstitute in sterile PBS buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.
Stability & Storage:24 months, -20 to -70 °C, under powder state; 12 months, -20 to -70 °C, under sterile conditions after reconstitution; 2 month, 2 to 8 °C under sterile conditions after reconstitution; avoid repeated freeze-thaw cycles.
