Organoids refer to tissue analogues with a certain spatial structure formed by three-dimensional cultivation of adult stem cells or pluripotent stem cells in vitro. Organs are highly consistent with their source tissues in terms of tissue structure, cell type, self-renewal ability, and function, thus demonstrating unique advantages in multiple biomedical fields such as developmental biology, disease modeling, precision medicine, drug research and development, gene and cell therapy, infection and immunity, and regenerative medicine.
Product Description:
Background:FGF-7 (fibroblast growth factor-7) is one of 22 known members of the mouse FGF family of secreted proteins that plays a key role in development, morphogenesis, angiogenesis, wound healing, and tumorigenesis.FGF-7 promotes cell migration and invasion, and mediates melanocyte transfer to keratinocytes upon UVB radiation. It has been used ectopically to avoid chemotherapy-induced oral mucositis in patients with hematological malignancies. Deletion of FGF-7 affects kidney development, producing abnormally small ureteric buds and fewer nephrons. It also impedes hair follicle differentiation .The 194 amino acid (aa) FGF-7 precursor contains a 31 aa signal sequence and, like all other FGFs, an ~120 aa beta -trefoil scaffold that includes receptor- and heparin-binding sites.
Product parameters
Source:E.coli
Protein Construction:A DNA sequence encoding the amino acids (Pro143-Ser288) of human FGF-2 (Accession Number:P48798) was expressed.
Synonyms:Basic fibroblast growth factor; bFGF; FGF basic; FGF-2.
SPECIFICATIONS
Purity:≥ 97%, by SDS-PAGE visualized with quantitative densitometry by Coomassie® Blue Staining.
Biological Activity:Measured in CellTiter-Glo 3D Cell Viability Assay using human bronchoalveolar organoids. The ED50 for this effect is 10-50 ng/ml.
Endotoxin Level:<0.10 EU per 1 μg of the protein by the LAL method
Calculated Molecular Weight:16.5 kDa
SDS-PAGE:18 kDa, reducing conditions
FORMULATION AND STORAGE
Formulation:The product is Lyophilized from a 0.22 μm filtered solution in PBS.
Shipping:The product is shipped on ice. Upon receipt, store it immediately as methods recommended below.
Reconstitution:Reconstitute in sterile PBS buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.
Stability & Storage:
24 months, -20 to -70 °C, under powder state;
12 months, -20 to -70 °C, under sterile conditions after reconstitution;
2 month, 2 to 8 °C under sterile conditions after reconstitution;
avoid repeated freeze-thaw cycles.
