• Organoid  Recombinant Human R-spondin1
  • Human Liver Ductal Organoid Kit (Differentiation)
  • Human Liver Ductal Organoid Kit (Differentiation)
  • Organoid  Recombinant Human R-spondin1
  • Human Liver Ductal Organoid Kit (Differentiation)
  • Human Liver Ductal Organoid Kit (Differentiation)

Organoid Recombinant Human R-spondin1

R-Spondin1 (Rspo-1) belongs to the (Rspo) family of Wnt modulators. Currently, the family consists of four structurally related secreted ligands (Rspo 1-4), all containing the furin-like and thrombospondin structural domains. Postnatally, R-Spondin1 is expressed by neuroendocrine cells in the intestine, adrenal gland and pancreas, and by epithelia in kidney and prostate. R-Spondin1 regulates Wnt/β-catenin by competing with the Wnt antagonist DKK-1 for binding to the Wnt coreceptors, Kremen and LRP-6, reducing their DKK-1-mediated internalization. Recombinant Human R-Spondin1 is a 27.3 kDa protein consisting of 233 amino acid residues. Due to glycosylation, RSpondin1 migrates at an apparent molecular weight of approximately 41.0 kDa by SDS-PAGE analysis under reducing conditions.
  • Organoid  Recombinant Human R-spondin1
  • Human Liver Ductal Organoid Kit (Differentiation)
  • Human Liver Ductal Organoid Kit (Differentiation)

SPECIFICATION

Organoids refer to tissue analogues with a certain spatial structure formed by three-dimensional cultivation of adult stem cells or pluripotent stem cells in vitro. Organs are highly consistent with their source tissues in terms of tissue structure, cell type, self-renewal ability, and function, thus demonstrating unique advantages in multiple biomedical fields such as developmental biology, disease modeling, precision medicine, drug research and development, gene and cell therapy, infection and immunity, and regenerative medicine.

Product Description:

Background:
R-Spondin1 (Rspo-1) belongs to the (Rspo) family of Wnt modulators. Currently, the family consists of four structurally related secreted ligands (Rspo 1-4), all containing the furin-like and thrombospondin structural domains. Postnatally, R-Spondin1 is expressed by neuroendocrine cells in the intestine, adrenal gland and pancreas, and by epithelia in kidney and prostate. R-Spondin1 regulates Wnt/β-catenin by competing with the Wnt antagonist DKK-1 for binding to the Wnt coreceptors, Kremen and LRP-6, reducing their DKK-1-mediated internalization. Recombinant Human R-Spondin1 is a 27.3 kDa protein consisting of 233 amino acid residues. Due to glycosylation, RSpondin1 migrates at an apparent molecular weight of approximately 41.0 kDa by SDS-PAGE analysis under reducing conditions.

Product instructions

Product parameters
Source:Chinese Hamster Ovary cell line

Protein Construction:A DNA sequence encoding the amino acids (Ala31-Ser263) of human Rspo-1 (Accession Number: Q2MKA7) was expressed.

Synonyms:Roof plate-specific spondin-1, RSPO1

Purity:≥ 95%, by SDS-PAGE visualized with quantitative densitometry by Coomassie® Blue Staining.

Biological Activity:Measured by its ability to induce activation of β-catenin response in a Top-flash Luciferase assay using HEK293T human embryonic kidney cells. The ED50 for this effect is typically 0.1-0.5 μg/ml in the presence of 5 ng/ml recombinant mouse Wnt3a.

Endotoxin Level:<0.10 EU per 1 μg of the protein by the LAL method

Calculated Molecular Weight:27.3 kDa

SDS-PAGE:41.0 kDa, reducing condition

Formulation:

The product is Lyophilized from a 0.22μm filtered solution in PBS.

Shipping:

The product is shipped on ice. Upon receipt, store it immediately as methods recommended below.

Reconstitution:

Reconstitute in sterile PBS buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Stability & Storage:

24 months, -20 to -70 °C, under powder state; 12 months, -20 to -70 °C, under sterile conditions after reconstitution; 2 month, 2 to 8 °C under sterile conditions after reconstitution; avoid repeated freeze-thaw cycles.



 

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