• Organoid  Red Blood Cell Lysis Solution
  • Human Liver Ductal Organoid Kit (Differentiation)
  • Human Liver Ductal Organoid Kit (Differentiation)
  • Organoid  Red Blood Cell Lysis Solution
  • Human Liver Ductal Organoid Kit (Differentiation)
  • Human Liver Ductal Organoid Kit (Differentiation)

Organoid Red Blood Cell Lysis Solution

Red Blood Cell Lysis Solution is a mild type of red blood cell lysate that utilizes the principle of cell membrane dilation caused by the difference in salt ion concentration inside and outside cells to lyse nucleated red blood cells. This product has undergone aseptic treatment and is mainly used for the separation and purification of tissue cells dispersed by enzyme digestion, the separation and purification of lymphocytes, and the removal of red blood cells in experiments such as tissue cell protein and nucleic acid extraction.
$60.00
  • Organoid  Red Blood Cell Lysis Solution
  • Human Liver Ductal Organoid Kit (Differentiation)
  • Human Liver Ductal Organoid Kit (Differentiation)

SPECIFICATION

Organoids refer to tissue analogues with a certain spatial structure formed by three-dimensional cultivation of adult stem cells or pluripotent stem cells in vitro. Organs are highly consistent with their source tissues in terms of tissue structure, cell type, self-renewal ability, and function, thus demonstrating unique advantages in multiple biomedical fields such as developmental biology, disease modeling, precision medicine, drug research and development, gene and cell therapy, infection and immunity, and regenerative medicine.

Product Description:

Red Blood Cell Lysis Solution is a mild type of red blood cell lysate that utilizes the principle of cell membrane dilation caused by the difference in salt ion concentration inside and outside cells to lyse nucleated red blood cells. This product has undergone aseptic treatment and is mainly used for the separation and purification of tissue cells dispersed by enzyme digestion, the separation and purification of lymphocytes, and the removal of red blood cells in experiments such as tissue cell protein and nucleic acid extraction.

Instructions for use
Fresh whole blood sample:
1. Add one volume of fresh whole blood and three volumes of red blood cell lysate. Add 3mL of red blood cell lysate to 1mL of fresh whole blood, gently swirl or invert and mix well.
2. Place on ice for 15 minutes, gently vortex and mix twice during this time. After the red blood cells lyse, the solution should be clear and transparent.
3. Collect cells: Centrifuge 450g at 4 ℃ for 10 minutes to precipitate white blood cells, and carefully discard the supernatant.
4. Add twice the initial blood volume of red blood cell lysate to the white blood cell precipitate, gently vortex and fully resuspend the white blood cells. If the initial blood volume is 1mL, add 2mL of red blood cell lysate.
5. Centrifuge at 450 g at 4 ℃ for 10 minutes to precipitate white blood cells, carefully and thoroughly remove the supernatant.
6. Resuspension cells for subsequent experiments; If RNA is extracted, it is best to start using a solution prepared with DEPC water at this step.
Fresh tissue samples:
1. Fresh tissue is digested and dispersed into a tissue cell suspension by collagenase and protease (or tumor tissue digest K601003), and the supernatant is discarded by centrifugation. 1mL of red blood cell lysate is added to the precipitate, and gently swirled or inverted to mix well.
2. Collect cells: Centrifuge 350g at 4 ℃ for 5 minutes to precipitate tissue cells, and carefully discard the supernatant.
3. Resuspension cells using PBS or basic culture medium for subsequent experiments.
Precautions:
This lysate is a sterile product. Please pay attention to the sterile operation when separating cells for cell culture. For your safety and health, please wear laboratory clothes and disposable gloves for operation.

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