• PCR Optimization Kit
  • PCR Optimization Kit

PCR Optimization Kit

This kit provides you with 8 types of heat-resistant polymerase and corresponding carefully prepared buffer solutions. The pH value, salt ion concentration, and cosolvent composition of the buffer solutions vary.
$8.50
  • PCR Optimization Kit

SPECIFICATION

                             PCR Optimization Kit
This kit provides you with 8 types of heat-resistant polymerase and corresponding carefully prepared buffer solutions. The pH value, salt ion concentration, and cosolvent composition of the buffer solutions vary. When problems arise with the amplification of certain complex gene templates, different polymerase and reaction buffer systems should be tested. In addition, this kit also provides PCR enhancers that can increase the specificity of primers, improve the thermal stability of DNA polymerase, and adapt to a wide range of annealing temperatures and Mg2+concentrations. PCR enhancers can be added to the 8 types of polymerase and corresponding reaction systems provided in the kit to find the optimal reaction system for PCR and achieve the best PCR reaction effect.
Why did you choose this reagent kit?
There are many factors that affect the PCR reaction, such as the properties of the polymerase itself, pH value, enzyme protectants, co solvents, etc. Only when these factors are all optimal can better amplification products be obtained.
Kit composition:
Component specifications
Taq DNA polymerase (5U/ μ l) 20 μ L
five × Taq Buffer with Mg2+1ml
UltraTaq DNA Polymerase (5U/ μ l) 20 μ L
five × UltraTaq Buffer with Mg2+1ml
Taq Plus DNA Polymerase (5U/ μ l) 20 μ L
five × Taq Plus Buffer with Mg2+1ml
UltraTaq Plus enzyme (5U/ μ l) 20 μ L
five × UltraTaq Plus Buffer with Mg2+1ml
Pfu DNA polymerase (5U/ μ l) 20 μ L
ten × Pfu Buffer with Mg2+1ml
Fast Pfu DNA Polymerase (5U/ μ l) 20 μ L
five × Fast Pfu Buffer with Mg2+1ml
two × Hot Start Taq PCR MasterMix 500 μ L
two × Hot Start Taq Plus PCR MasterMix 500 μ L
PCR enhancer I 200 μ L
PCR enhancer II 200 μ L
The reaction speed of various PCR polymerase enzymes:
PCR polymerase reaction rate
Taq DNA polymerase 1kb/60 seconds
UltraTaq DNA polymerase 4kb/60 seconds
Taq Plus DNA polymerase 1kb/60 seconds
UltraTaq Plus enzyme 4kb/60 seconds
Pfu DNA polymerase 0.5kb/60 seconds
Fast Pfu DNA polymerase 2kb/60 seconds
Hot Start Taq DNA polymerase 1kb/60 seconds
Hot Start Taq Plus DNA Polymerase 1
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