• Plant direct PCR Kit(without extraction of DNA)
  • Plant direct PCR Kit(without extraction of DNA)

Plant direct PCR Kit(without extraction of DNA)

This reagent kit uses a unique lysis buffer to quickly lyse plant tissue, releasing DNA that can be used as a template without purification. It is highly resistant to inhibitors using 2 × Direct PCR Mix amplification allows for direct sample electrophoresis of the amplified products.
$17.00
  • Plant direct PCR Kit(without extraction of DNA)

SPECIFICATION

         Plant direct PCR Kit(without extraction of DNA)
This reagent kit uses a unique lysis buffer to quickly lyse plant tissue, releasing DNA that can be used as a template without purification. It is highly resistant to inhibitors using 2 × Direct PCR Mix amplification allows for direct sample electrophoresis of the amplified products. Suitable for simple non polysaccharide and polyphenolic plants. Easy to operate, high-throughput screening; The general amplification size is less than 2kb.
Kit components (100 times):
Stock A - - - - - -0.5mL
Stock B - - - - - -1mL
Buffer N -- -- -- -- --5mL
two × Direct PCR Mix -1mL
Double distilled water (PCR grade) - - -10mL
Precautions:
If the amplification band is weak, the amount of template added can be appropriately increased or reduced, and the template for cracking mixture can generally be within 0.5 μ L-2 μ Adjust within L, but note that it should generally not exceed 4 μ L. Adding too much actually suppresses the PCR reaction.
2. If the sample contains a large amount of polyphenol polysaccharides and the amplification effect is not ideal, it can be amplified within 20 μ Addition of L PCR reaction system 2 μ Improvement effect of L 10% PVP40.
If there are many non-specific amplifications, the annealing temperature can be adjusted, or the amount of template can be appropriately increased or decreased.
4. After using each solution, the lid should be immediately closed to prevent pH changes from affecting the quality.
Storage conditions: -20 ℃.
Nickname of this product: Plant DNA Free Extraction Direct PCR Kit
CONTACT US
FirstName*
LastName*
Email*
Message*