Product Information:
Component |
Component Cat# |
Volume |
Storage& Stability |
Prostate Cancer Organoid Kit Prostate Cancer Organoid Kit |
K2006-MLH –A100/A500 |
100mL/500mL |
4℃,12 months |
Prostate Cancer Organoid Supplement B(50x)(Differentiation) |
K2006-MLH –B100/B500 |
2mL/10mL |
-20℃,avoid repeated freeze-thaw cycles, 12 months |
Prostate Cancer Organoid Supplement C(250x) (Differentiation) |
K2006-MLH –C100/C500 |
0.4mL/2mL |
-20℃, avoid repeated freeze-thaw cycles, 12 months |
Prostate Cancer Organoid Supplement D(250x) (Differentiation) |
K2006-MLH –D100/D500 |
0.4mL/2mL |
-20℃, avoid repeated freeze-thaw cycles, 12 months |
Preparation of Prostate Cancer Organoid Differentiation Medium
Use sterile technique to prepare theProstate Cancer organoid differentiation medium. mLDs grown inProstate Cancer Organoid Expansion Medium overwhelmingly consisted of cholangiocytes. After changing the Expansion Medium to differentiation medium, the mLDs could differentiate into hepatocyte like cells, which display the markers of hepatocytes, including Albumin, Ttr, Cyp3a11 and Mup20. The following examples are for preparing 10 mL of Differentiation I Medium and Differentiation II Medium. If preparing other volumes, adjust accordingly.
1. Thaw Prostate Cancer Organoid Supplement B(50x) (Differentiation),Prostate Cancer Organoid Supplement C(250x) (Differentiation) andProstate Cancer Organoid Supplement D(250x) (Differentiation) on ice.
NOTE: Once thawed, use immediately or aliquot and store at -20°C for no more than 10 months. After thawing the aliquots, use immediately. Do not re-freeze.
2. For Prostate Cancer Organoid Differentiation Medium I. Add 200 μLProstate Cancer Organoid Supplement B(50x) (Differentiation), 40 μLProstate Cancer Organoid Supplement C(250x) (Differentiation) to 10 mLProstate Cancer Organoid Basal Medium (Differentiation). Mix thoroughly.
3. For Prostate Cancer Organoid Differentiation Medium II. Add 200 μLProstate Cancer Organoid Supplement B(50x) (Differentiation)and 40 μLProstate Cancer Organoid Supplement C(250x) (Differentiation) and 10 μLProstate Cancer Organoid Supplement D (250x) (Differentiation) to 10 mLProstate Cancer Organoid Basal Medium (Differentiation). Mix thoroughly.
NOTE: If not use immediately, store complete medium at 2-8°C for no more than 2 weeks. bioGenousTMProstate Cancer Organoid Supplement B (Differentiation) contains fungicide and antibiotics(50x).
Protocol for Prostate Cancer Organoids Differentiation
1. Culture the mouse ductal organoids inProstate Cancer Organoid Medium(Expansion) (K2006-MLD) for at least 3 days.
2. Change the medium toProstate Cancer Organoid Differentiation Medium I, and culture for 9 days. During this period, replace the medium every day.
3. Change the medium toProstate Cancer Organoid Differentiation Medium II, and culture for 3 days. During this period, replace the medium every day.
4. At the end of this period, the differentiation process is completed. The ductal organoid would differentiate into hepatocyte-like organoid with the expression of hepatic markers, such as Albumin, Ttr, Cyp3a11 and Mup20.