• Prostate Cancer Organoid Kit
  • Nasopharyngeal Carcinoma Organoid Kit
  • Human Liver Ductal Organoid Kit (Differentiation)
  • Human Liver Ductal Organoid Kit (Differentiation)
  • Prostate Cancer Organoid Kit
  • Nasopharyngeal Carcinoma Organoid Kit
  • Human Liver Ductal Organoid Kit (Differentiation)
  • Human Liver Ductal Organoid Kit (Differentiation)

Prostate Cancer Organoid Kit

Prostate Cancer Organoid Kit is a chemically defined cell culture medium for the establishment and
maintenance of human Prostate Cancer organoids. The bioGenousTM Prostate Cancer Kit is specially formulated forthe in vitro culture of a small piece of tissue or a few cells obtained at any stage of tumor development. Patient-derivedcancer organoids recapitulate the genomic and pathological features of original tumors and therefore hold great promise for medical research and precision medicine.
$850.00
  • Prostate Cancer Organoid Kit
  • Nasopharyngeal Carcinoma Organoid Kit
  • Human Liver Ductal Organoid Kit (Differentiation)
  • Human Liver Ductal Organoid Kit (Differentiation)

SPECIFICATION

Prostate Cancer Organoid Kit is a chemically defined cell culture medium for the establishment and
maintenance of human Prostate Cancer organoids. The bioGenousTM Prostate Cancer Kit is specially formulated forthe in vitro culture of a small piece of tissue or a few cells obtained at any stage of tumor development. Patient-derivedcancer organoids recapitulate the genomic and pathological features of original tumors and therefore hold great promise for medical research and precision medicine.

Product Information:

Component

Component Cat#

Volume

Storage& Stability

Prostate Cancer Organoid Kit Prostate Cancer Organoid Kit

K2006-MLH –A100/A500

100mL/500mL

4℃,12 months

Prostate Cancer   Organoid Supplement B(50x)(Differentiation)

K2006-MLH –B100/B500

2mL/10mL

-20℃,avoid repeated freeze-thaw cycles, 12 months

Prostate Cancer   Organoid Supplement C(250x) (Differentiation)

K2006-MLH –C100/C500

0.4mL/2mL

-20℃, avoid repeated freeze-thaw cycles, 12 months

Prostate Cancer   Organoid Supplement D(250x) (Differentiation)

K2006-MLH –D100/D500

0.4mL/2mL

-20℃, avoid repeated freeze-thaw cycles, 12 months

 

Preparation of Prostate Cancer   Organoid Differentiation Medium
Use sterile technique to prepare theProstate Cancer   organoid differentiation medium. mLDs grown inProstate Cancer   Organoid Expansion Medium overwhelmingly consisted of cholangiocytes. After changing the Expansion Medium to differentiation medium, the mLDs could differentiate into hepatocyte like cells, which display the markers of hepatocytes, including Albumin, Ttr, Cyp3a11 and Mup20. The following examples are for preparing 10 mL of Differentiation I Medium and Differentiation II Medium. If preparing other volumes, adjust accordingly.
1.      Thaw Prostate Cancer   Organoid Supplement B(50x) (Differentiation),Prostate Cancer   Organoid Supplement C(250x) (Differentiation) andProstate Cancer   Organoid Supplement D(250x) (Differentiation) on ice.
NOTE: Once thawed, use immediately or aliquot and store at -20°C for no more than 10 months. After thawing the aliquots, use immediately. Do not re-freeze.
2.       For Prostate Cancer   Organoid Differentiation Medium I. Add 200 μLProstate Cancer   Organoid Supplement B(50x) (Differentiation), 40 μLProstate Cancer   Organoid Supplement C(250x) (Differentiation) to 10 mLProstate Cancer   Organoid Basal Medium (Differentiation). Mix thoroughly.
3.       For Prostate Cancer   Organoid Differentiation Medium II. Add 200 μLProstate Cancer   Organoid Supplement B(50x) (Differentiation)and 40 μLProstate Cancer   Organoid Supplement C(250x) (Differentiation) and 10 μLProstate Cancer   Organoid Supplement D (250x) (Differentiation) to 10 mLProstate Cancer   Organoid Basal Medium (Differentiation). Mix thoroughly.
NOTE: If not use immediately, store complete medium at 2-8°C for no more than 2 weeks. bioGenousTMProstate Cancer   Organoid Supplement B (Differentiation) contains fungicide and antibiotics(50x). 

Protocol for Prostate Cancer   Organoids Differentiation

1.     Culture the mouse ductal organoids inProstate Cancer   Organoid Medium(Expansion) (K2006-MLD) for at least 3 days.
2.     Change the medium toProstate Cancer   Organoid Differentiation Medium I, and culture for 9 days. During this period, replace the medium every day.
3.     Change the medium toProstate Cancer   Organoid Differentiation Medium II, and culture for 3 days. During this period, replace the medium every day.
4.     At the end of this period, the differentiation process is completed. The ductal organoid would differentiate into hepatocyte-like organoid with the expression of hepatic markers, such as Albumin, Ttr, Cyp3a11 and Mup20.

CONTACT US
FirstName*
LastName*
Email*
Message*