• PTrc Taq polymerase gene plasmid
  • PTrc Taq polymerase gene plasmid

PTrc Taq polymerase gene plasmid

1. After receiving the plasmid dry powder, please centrifuge at 5000rpm for 1 minute before adding 20 μ Dissolve the plasmid in sterile water and let it sit at room temperature for 1 minute; 2. Remove the corresponding receptive state from the -80 ℃ refrigerator, thaw it on an ice box, and mark it; 3. Take 2 μ Add plasmid to 100 μ In the receptive state, ice bath for 30 minutes; 4. 42 ℃ heat shock for 90 seconds, then ice bath for 2 minutes;
$150.00
  • PTrc Taq polymerase gene plasmid

SPECIFICATION

Basic information
Promoter: Trc/lac
Terminator: rrnB T1, rrnB T2
Plasmid classification: protease expression plasmids
Plasmid label: Taq
Prokaryotic resistance: Ampicillin
Clone strain: DH5 α
Culture conditions: 37 ℃, aerobic, LB
Expression host: Escherichia coli
Culture conditions: 37 ℃, aerobic, LB
Induction method: IPTG and lactose analogues
5 'sequencing primer: designed based on Taq sequence

Instructions for using the rc-Taq polymerase gene plasmid:
1. After receiving the plasmid dry powder, please centrifuge at 5000rpm for 1 minute before adding 20 μ Dissolve the plasmid in sterile water and let it sit at room temperature for 1 minute;
2. Remove the corresponding receptive state from the -80 ℃ refrigerator, thaw it on an ice box, and mark it;
3. Take 2 μ Add plasmid to 100 μ In the receptive state, ice bath for 30 minutes;
4. 42 ℃ heat shock for 90 seconds, then ice bath for 2 minutes;
5. Join 900 μ L Antibiotic free LB liquid culture medium, shaken at 180 rpm for 45 minutes;
6. Centrifuge at 6000rpm for 5 minutes, leaving only 100ul of supernatant to mix well with bacterial precipitation;
7. Add the mixed bacterial solution to the LB plate with corresponding resistance, pour an appropriate amount of glass beads, and apply the liquid evenly;
8. Incubate the plate in a positive direction for 1 hour, then invert it for 12-16 hours;
9. Select monoclonal colonies and place them in LB liquid culture medium with corresponding resistance. Shake and culture for 12-16 hours, and extract plasmids according to experimental needs.

Precautions for pTrc Taq polymerase gene plasmid:
1. If you have received a glycerol strain, please mark the four zones first and select monoclonal clones for cultivation.
2. If the conversion plate becomes too long the next day, please dilute the plasmid proportionally before conversion.
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