PureDireX Plasmid miniPREP Kit (PDP01-0100)
The PureDireX Plasmid miniPREP Kit provides a fast, simple, and cost-effective method for isolation of plasmid DNA from cultured Gram-positive and Gram-negative bacterial cells.
Principle
The design is based on alkaline lysis of bacterial cells, followed by binding of DNA onto the glass fiber matrix of the spin column in the presence of highly concentrated salts. Plasmid DNA purified with this kit is suitable for a variety of routine applications.
Specifications
Catalog No.: PDP01-0100
Sample input: Up to 4 ml bacterial cells
Yield: Up to 30 μg of plasmid DNA
Procedure time: 15–20 minutes (fastest procedure)
Sample type: Gram-positive and Gram-negative bacterial cells
Key Features
Fastest procedure: Entire workflow completed within 15–20 minutes.
Broad sample compatibility: Works with both Gram-positive and Gram-negative bacterial cells.
Cost-effective: Simple spin-column format for routine plasmid isolation.
Reliable downstream performance: Purified plasmid DNA is suitable for restriction enzyme digestion, sequencing, library screening, in vitro translation, transfection of robust cells, ligation, and transformation.
Applications
Restriction enzyme digestion, sequencing, library screening, in vitro translation, transfection of robust cells, ligation, and transformation.
