Name: Fluorescent Master Mix
Purity: ≥ 90%
Delivery time: 24 hours
Product number: BT-LA5203-01
Specification: 100 rxn
Product Description
This product contains RT Enzymes Mix for reaction buffer, BstL DNA polymerase, and heat-resistant reverse transcriptase, freeze-drying protectants, and fluorescent dye components. The reaction buffer contains Mg2+, dNTP and other necessary components for amplification. When using it, only the Buffer, reaction enzyme, fluorescent dye, primer need to be mixed and added to the template; If a freeze-drying protective agent is added, a freeze-drying system can be further configured.
Application: Suitable for isothermal amplification of DNA and RNA. Transportation and storage method: Dry ice transportation, stored at -25~-15 ℃. Avoid repeated freezing and thawing, and the product is valid for 12 months.
Experimental process
1) Remove the buffer and thaw it. Before use, thoroughly vortex and mix, and briefly centrifuge to collect the liquid to the bottom of the tube.
2) The reaction system is prepared according to the following table
Remarks:
ten × Primer Mix concentration: 16 μ M FIP/BIP,
two μ M F3/B3, 4 μ M Loop F/B. The nucleic acid template is recommended to be dissolved in DEPC water.
3) Amplification reaction: Set the following program on a fluorescence PCR instrument (such as ABI7500).
Matters needing attention
1) After the buffer melts, there may be white precipitates, which can be clarified by thoroughly mixing at room temperature.
2) The reaction temperature can be optimized between 62 ℃ and 68 ℃ based on the primer situation.
3) The experiment should be operated in a standardized manner, including the preparation of reaction systems, sample processing, and sample addition processes. To avoid pollution, it is recommended to prepare a reaction system in the ultra clean table and add templates in the fume hoods of other rooms to avoid false positive interference.